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pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

Plasmid

#119074

Purpose

Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:MCP-p65-HSF1-2a-Cerulean

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish ubiquitin promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

Plasmid

#119075

Purpose

Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:Com-KRAB-2a-Cerulean

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish ubiquitin promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-MS2-Ds

Plasmid

#119076

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xMS2-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET3a-Yuh1

Plasmid

#18895

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
enz.083-NMT1

Plasmid

#154047

Purpose

Expresses NMT1 in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

NMT1 (N-myristoyltransferase 1) (NMT1 Human)

Use

Tags

His, MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRBBm34

Plasmid

#48114

Purpose

Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3833

Plasmid

#47839

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 8 and BCD1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pKB01-PssbB-mKate2

Plasmid

#69105

Purpose

Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mKate2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pSUMO-LIC-CHIKV-nsP2h

Plasmid

#127935

Purpose

Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

Depositor

Dahai Luo

Article

Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

Insert

CHIKV nsP2h

Use

Tags

His6-Sumo-TEV

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PTK2BA

Plasmid

#42401

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTK2BA (PTK2B Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-KG-CAND1

Plasmid

#19949

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
p2B-57

Plasmid

#177927

Purpose

Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)

Depositor

Vadim Gladyshev

Article

Barre et al (unpublished)

Insert

EGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TET3 (contains three rtTA binding sites)

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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RFP-(SIM)1

Plasmid

#127094

Purpose

Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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Reb_81 aka PAS622

Plasmid

#71227

Purpose

Reb_1 with RebB(T28A, D72G)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Use

Tags

none

Expression

Bacterial

Mutation

RebB(T28A, D72G), RebA(G53A)

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pKM402

Plasmid

#107770

Purpose

Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Inserts

Che9C RecT
TetR (tet repressor)
cat
kan

Use

Tags

Expression

Bacterial

Mutation

Promoter

Cat promoter, Ptet, kan promoter, and pTB21

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSiMPla_N + pSiMPla_C

Plasmid

#134314

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

ampR_gp41-1 N-intein + ampR_gp41-1 C-intein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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NM-01

Plasmid

#69799

Purpose

Bacterial expression of Cas9 with His, MBP, and Strep tags

Depositor

Gerard Cagney

Insert

Cas9

Use

Tags

HIS, MBP, and StrepII

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only
pBbS9a-RFP

Plasmid

#126172

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23113

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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