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Plasmid#31396DepositorInsertpJT122
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-PCNA
Plasmid#105977Purposeentry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNADepositorInsertPCNA (PCNA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-phzAG
Plasmid#141106PurposeContains a seven gene phzABCDEFG operon BioBrickDepositorArticleInsertphzABCDEFG
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046955
Plasmid#145616PurposeBacterial Expression plasmid for RNA-dependent RNA polymeraseDepositorInsertRNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC029 - Lw2Cas13a from Leptotrichia wadei F0279
Plasmid#91919PurposeExpresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLw2Cas13a and guide expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pA5c-tesA-vhb50-8fadR
Plasmid#86601Purposeexpression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoterDepositorInsertstesA
Vhb50
fadR
UseTagsExpressionBacterialMutationPromoterLacUV5 and pBADAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_A
Plasmid#168051PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
UseTagsExpressionBacterialMutationPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTn7(Gm)PA1/04/03-eyfp-a
Plasmid#111620PurposeeYFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-eyfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GatA_pET29b
Plasmid#215406PurposeExpression construct for GatA gene, codon optimized for expression in E. coliDepositorInsertGatA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RUSC1-1/1
Plasmid#91379PurposeProtein expression and purification of human SH3 domain construct RUSC1-1/1DepositorInsertRUSC1-1/1 (RUSC1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HCK-1/1
Plasmid#91273PurposeProtein expression and purification of human SH3 domain construct HCK-1/1DepositorInsertHCK-1/1 (HCK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP325
Plasmid#159596PurposeExpress E.coli TonB proteinDepositorInserttonB
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-3WJdB-T
Plasmid#87311PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.DepositorInsertThree-way Junction dimeric Broccoli aptamer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBC13-PGEX4T
Plasmid#25464DepositorUseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
AARS_C1_pNIC-Bio3
Plasmid#153046PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorInsertAARS (AARS Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Met1 diUb (1-152)
Plasmid#110766PurposeBacterial expression of Met1 diUbDepositorInsertUBB (UBB Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2T
Plasmid#15808DepositorInsertUbiquitin conjugating enzyme UbE2T (UBE2T Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-hRanGAP1
Plasmid#50941Purposeexpression of full length untagged human RanGAP1 in E.coliDepositorInsertRanGTPase activating protein 1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only