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Plasmid#71486PurposeContains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.DepositorInsertloxP-hyg-loxP cassette
UseSource of loxp-hyg-loxp for pcr amplification.TagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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PET-eSpCas9-plus-NLS-6xHis
Plasmid#207380PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9-plus
UseCRISPRTags6xHisExpressionBacterialMutationK848A, R1060A, amino acids 1005-1013 replaced wit…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
EHF_pet28a
Plasmid#131660PurposeBacterial expression of ETS gene EHFDepositorInsertEHF (EHF Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6B (full-length, aa 1-293)
Plasmid#61418PurposeExpresses human OTUD6B (full-length) in E. coli.DepositorInsertOTUD6B (OTUD6B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-EGFP_opt
Plasmid#106334PurposeGateway Entry vector for EGFP (codon optimized for efficient gene synthesis)DepositorInsertEGFP
UseGateway entry vectorTagsExpressionBacterial and MammalianMutationcodon optimized for gene synthesis without affect…PromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-NusA
Plasmid#112571PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJKR-L-mphR
Plasmid#62560PurposeMacrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSG291
Plasmid#115605Purposesurface-displayed Null, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Klenow-ST2-com-vector
Plasmid#176235PurposeVector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRc20_992
Plasmid#49739DepositorInsertAS20_992
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ4M_hnRNPA2_FL_D290V
Plasmid#139110PurposeExpress FL hnRNPA2 D290V with a C-terminal MBP tagDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationD290VPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Eptesicus fuscus
Plasmid#78113PurposeNt His-tag Eptesicus fuscus Sacsin 89-1368DepositorInsertSacsin Eptesicus fuscus 89-1368
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAKgfplux2
Plasmid#14084DepositorInsertBacterial luciferase-lacIq repressor gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
MARS_pNIC-CTB10H
Plasmid#153053PurposeExpression construct for producing Avi tagged Methionyl-tRNA synthetaseDepositorInsertMARS (MARS1 Human)
UseTagsTEV-Avi-His10ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-LIC+
Plasmid#62312PurposeE. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage siteDepositorTypeEmpty backboneUseTags6xHis and thrombin protease cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-RAB17
Plasmid#34812DepositorInsertRAB17 (RAB17 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGV3340
Plasmid#170278PurposeExpression of mutant HIV-2 RT p51 reverse transcriptaseDepositorInsertHIV-2 RT p51 (mutant)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HaloTag7_dead_mt
Plasmid#167267PurposeOverexpression of the dead variant (D106A) of HaloTag7 in E. coliDepositorInsertHaloTag7
UseTagsHis x10ExpressionBacterialMutationD106APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only