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Plasmid#65119PurposeEncodes pHHF2 as a Type 2 part to be used in the Dueber YTK systemDepositorInsertpHHF2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK019
Plasmid#65126PurposeEncodes pPAB1 as a Type 2 part to be used in the Dueber YTK systemDepositorInsertpPAB1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK010
Plasmid#65117PurposeEncodes pCCW12 as a Type 2 part to be used in the Dueber YTK systemDepositorInsertpCCW12
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB035h
Plasmid#79191PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-HA4
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (5B)
Plasmid#85498PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-mF(3230)A3
Plasmid#168016PurposeExpresses MBP-F(3230)A3-6xHis in bacterial cellsDepositorInsertMBP-F(3230)A3-6xHis
UseTagsHisx6 and maltose binding proteinExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80l A-CC-A
Plasmid#196478PurposeProtein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELPDepositorInsertA-CC-A
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-K128N
Plasmid#120284PurposeProduce human recombinant FGF2 K128N in DE3 cellsDepositorInsertBasic fibroblast growth factor 2 (FGF2 Synthetic, Human)
UseTags6x HisExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)
Plasmid#159687PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)DepositorInsertHis6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆repeats-mNeonGreen-IRESpuro2
Plasmid#183742PurposeExpresses Ki67∆repeats-mNeonGreenDepositorInsertMKI67(lacking repeats) (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking repeats)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD25Q
Plasmid#11508DepositorInserthuntingtin, exon 1, 25 glutamines (HTT Human)
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 25 glutamines, vector m…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSG84-pLas-TetRssrA
Plasmid#102455PurposeExpresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.DepositorInsertTetR (tetR )
UseTagsssrAExpressionBacterialMutationPromoterpLasAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG4
Plasmid#70115PurposeTn7-based lux reporter for promoter cloning in bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoternoneAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSSX-STC
Plasmid#115668PurposeE.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tagDepositorInsertsmall tetraheme cytochrome c
UseTagsSignal peptide (aa1-27)-Strep-II tag- Factor Xa c…ExpressionBacterialMutationPromotertacAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet42c(+)-GST-USP5
Plasmid#61750Purposeexpress N-terminally GST tagged human USP5DepositorInsertUSP5 (USP5 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP2846
Plasmid#160676PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
4MVT
Plasmid#60101PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPIAS3:YTC008-B10:C225910 (PIAS3 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSG020
Plasmid#126503PurposePseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHisDepositorInsertdisaggregase ClpGgi with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC201plus
Plasmid#164227PurposeIntegrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds
Plasmid#119059PurposeFluorescent vector with zebrafish pax3a enhancer 5 (strong activity)DepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only