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Plasmid#207374PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSniper SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationF539S, M763I, K890NPromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
rrsB-RFP
Plasmid#63838PurposeThis plasmid is designed as a bacterial nutrient sensorDepositorInsertrrsB, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is transcriptionally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CSK-1/1
Plasmid#91542PurposeProtein expression and purification of human SH3 domain construct CSK-1/1DepositorInsertCSK-1/1 (CSK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mVenus-I
Plasmid#163763PurposeExpresses Streptomyces codon-optimised mVenus-IDepositorInsertmVenus-I
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterSP44Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK
Plasmid#126928PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAF
Plasmid#104152PurposePlasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.DepositorInsertBarrier-to-autointegration factor (BAF) (BANF1 Human)
UseTagsN-terminal His tag with thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
UseTagsExpressionBacterialMutationPromoterE . coli insulated waaH gene promoter, low phosp…Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSty11
Plasmid#12401DepositorInsertSty11
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJKR-O-acuR
Plasmid#62569PurposeAcrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.DepositorInsertCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFD_R
Plasmid#198277PurposeFor loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genesDepositorInsertPdp1_NTD-ZFD_R
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PPL His6 MBP LIC cloning vector (2K-T)
Plasmid#37183DepositorTypeEmpty backboneUseTagsHis6, MBP, and PPLExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG-RGG
Plasmid#124938PurposeExpression of RGG-RGG-RGGDepositorInsertRGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKJ1712
Plasmid#21870DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal mKate2
Plasmid#104030PurposePlasmid encoding 2ndVal mKate2DepositorInsert2ndVal mKate2
UseLow copyTagsExpressionBacterialMutationmKate2 with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Top7 V48V
Plasmid#12393DepositorInsertTop7 V48V
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pConRef-2H12
Plasmid#221182PurposecdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)DepositorInsertscdGreen
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-MBP-TEV-GG
Plasmid#215800PurposeCloning of target gene with N terminal His6 MBP tag and TEV CleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6p-1-CYB5R3 deltaN26
Plasmid#204557PurposeExpresses GST-tagged CYB5R3 deltaN26 in E.coliDepositorInsertCYB5R3 (CYB5R3 Human)
UseTagsExpressionBacterialMutationdelta1-26PromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCJ206
Plasmid#162679PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
UseTagsHisExpressionBacterialMutationE226T; codon optimized for expression in E. coli …PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+10A)-IDR-mCherry
Plasmid#145282PurposeBacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion ProteinDepositorInsertHOXD13(+10A)-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only