-
Plasmid#67159Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS06
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationM374Q within GCaMP6fPromoterT7Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZP10 Snail
Plasmid#16931DepositorInsertsnail (snai1a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTmpGm
Plasmid#176229PurposeA template plasmid for amplification of the cI-hok-aacC4 cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, aacC4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTK164
Plasmid#13533DepositorInsertFLIPW-CTY
UseTagsECFP and VenusExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
AgAK
Plasmid#71290PurposeExpression of A. gambiae adenosine kinaseDepositorInsertAdenosine Kinase
UseTags6X HistidineExpressionBacterialMutationPromoterT7Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL5-pCMV7.1
Plasmid#155024PurposeN-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vectorDepositorInsertCullin 5 (CUL5 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYTK014
Plasmid#65121PurposeEncodes pTEF2 as a Type 2 part to be used in the Dueber YTK systemDepositorInsertpTEF2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxVenus
Plasmid#103993PurposePlasmid encoding moxVenusDepositorInsertmoxVenus
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16
Plasmid#153524PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#16DepositorInsertSb#16
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1360
Plasmid#119278Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProC.gfp
Plasmid#112517PurposeProC driven gfpmut3 expression with synTTDepositorInsertProC gfpmut3 TT
UseTagsExpressionBacterialMutationPromoterProCAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
UseTagsExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-154
Plasmid#118536PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBADmod1-linker2-gamS
Plasmid#45833DepositorInsertgamS
UseSynthetic BiologyTagsHistagExpressionBacterialMutationPromoterpBADAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-Drp1
Plasmid#72927PurposeBacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage siteDepositorInsertDrp1 (Dnm1l Mouse)
UseTags6xHis-tag and PreScission Protease siteExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-LexA
Plasmid#26258DepositorInsertLexA
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PprgH-GFPLVA
Plasmid#23340DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorInsertSNCA-GFP (SNCA Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG2b-srt-his
Plasmid#124804PurposeHDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.DepositorInsertMurine IgG2b
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only