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pH6HTC_mmHUR-Halo

Plasmid

#175311

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (Elavl1 Mouse)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT21

Plasmid

#200995

Purpose

pLv2-pUC18R6KT-miniTn7-Gm, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22

Plasmid

#200996

Purpose

pLv2-pUC18R6KT-miniTn7-Km, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT23

Plasmid

#200997

Purpose

pLv2-pUC18R6KT-miniTn7-Tc, TcR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT24

Plasmid

#200998

Purpose

pLv2-pUC18R6KT-miniTn7-aad9, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.Y

Plasmid

#201004

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23100

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT14.Y

Plasmid

#201009

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sYFP2, SpR,StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23100

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22.RY

Plasmid

#201018

Purpose

pLv1-pUC18R6KT-miniTn7-Km-mCherry-sYFP2, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22.YB

Plasmid

#201019

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sYFP2-TagBFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sYFP2
TagBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT20

Plasmid

#200990

Purpose

pL0V-Lv2-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pQTEV-GYG

Plasmid

#31324

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

glycogenin (GYG1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP709

Plasmid

#79986

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Use

Tags

Expression

Bacterial

Mutation

Promoter

araB

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET a sfGCaMP6s

Plasmid

#100022

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfGCaMP6s

Use

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJOE6090.1

Plasmid

#135080

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

Insert

P-rhaP-BAD-Strep-tag II-eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDZ585 pKAN 2x-mCherry

Plasmid

#72236

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

2x-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLS1

Plasmid

#31490

Depositor

Kathleen Matthews

Article

Swint-Kruse et al Protein Sci. 2001 Feb . 10(2):262-76.

Insert

lactose repressor protein (lacI )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pRiboI-Sec-APEX2m

Plasmid

#176845

Purpose

Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-Sec-APEX2m

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDJSVT86

Plasmid

#108965

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

Depositor

Daniel Slade

Article

Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

Type

Empty backbone

Use

Tags

OmpA 1-27, 6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pttq18

Plasmid

#69122

Purpose

regulated expression of genes in Escherichia coli

Depositor

Michael Stark

Article

Stark et al Gene. 1987;51(2-3):255-67.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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