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Plasmid#212129PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRhodoplanes bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM
Plasmid#212124PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBacteroidetes bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Runella-bGSDM
Plasmid#212123PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRunella bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM
Plasmid#212127PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertVitiosangium bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Gillisia-bGSDM
Plasmid#212128PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertGillisia bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bradyrhizobium-bGSDM
Plasmid#212126PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBradyrhizobium bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-dAsCas12f
Plasmid#204641PurposeExpression of an inactive AsCas12f in E. coliDepositorInsertinactive AsCas12f
UseTags6xHisExpressionBacterialMutationD225APromoterT7Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV SPOP 1-374
Plasmid#197015PurposeE. coli expression vectorDepositorInsertSPOP (Spop Mouse)
UseTags6His-SUMO-TEVExpressionBacterialMutationPromoterAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-GFP(S45C)-ZdK1
Plasmid#185791PurposeEncodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linkerDepositorInsertRBP-sfGFP(S45C)-ZdK1
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationSer to Cys mutation at start of GFP, residue 45 i…PromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA14_G-alpha
Plasmid#109914PurposeProtein expression and purification of GNA14_G-alphaDepositorInsertGNA14_G-alpha (GNA14 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDEST17-UbE2D4
Plasmid#15786DepositorInsertUbiquitin conjugating enzyme UbE2D4 (UBE2D4 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMK90
Plasmid#13323DepositorInsertgyrA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOTIII.EFGP.IGR3.6
Plasmid#40866DepositorInsertMOUSE OXYTOCIN GENE (Oxt Mouse)
UseTagsExpressionBacterialMutationPromotermouse oxytocin promoterAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-143
Plasmid#118527PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAB085c
Plasmid#79208PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); PtetAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HSPA9_HSP70
Plasmid#109776PurposeProtein expression and purification of HSPA9_HSP70DepositorInsertHSPA9_HSP70 (HSPA9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
UseTags3' extension derived from endogenous locus a…ExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only