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Plasmid#115598Purposesurface-displayed Nb3, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-de4GFP
Plasmid#85472PurposeInducible expression of dual emitting pH sensitive GFP in bacterial cellsDepositorInsertde4GFP
UseTags6xHisExpressionBacterialMutationS65T/C48S/H148C/T203CPromoterT7Available sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCORE-Kp53
Plasmid#72241PurposeContains the CORE cassette with KanMX4 and human p53 gene markersDepositorInsertsUseTagsExpressionBacterialMutationV122A mutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2-gfp
Plasmid#109290PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertGFP
UseTagsExpressionBacterialMutationWTPromoterKanamycinAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_TKT-Halo
Plasmid#175331PurposeProduction of HaloTag fusion proteinDepositorInsertTKT (TKT Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1-T-Sapphire
Plasmid#103977PurposePlasmid encoding T-SapphireDepositorInsertT-Sapphire
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (Q69M, C70V, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
DOT1L
Plasmid#36196PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDOT1L (GeneScript Synthesized) (DOT1L Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains amino acid residues 1-420PromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS1398
Plasmid#205971PurposeMWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1DepositorInsertCas5-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-G6
Plasmid#194589PurposeBacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertG6 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-Vif
Plasmid#46313DepositorInsertsp19
Vif DNA-1
linker
Vif DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)
Plasmid#159696PurposeE. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)DepositorInsertHis6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
UseTagsHis6-Halotag-tevExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PAmCherry-KanMX6
Plasmid#87026PurposeTag S. pombe genes in C terminal with PAmCherryDepositorInsertPAmCherry
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFAR5
Plasmid#149478PurposeBroad-Host range expression vector, RSF1010 origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertAraC-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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PJEBAN2
Plasmid#203681PurposeExpresses Cyan Fluorescent Protein (CFP) in Listeria monocytogenesDepositorInsertCyan Fluorescent Protein (CFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP2822
Plasmid#160670PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB082h
Plasmid#79240PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d23C
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_01
Plasmid#194662PurposeProtein production of the split HaloTag based calcium recorder Caprola_01 in bacteriaDepositorInsertCaprola_01
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)
Plasmid#159539PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)DepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only