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pDonor14-AcHELIX-KanR (CJT96)
Plasmid#181794PurposepDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Stu2 1-590
Plasmid#38314DepositorInsert5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
EPB41L3A-c000
Plasmid#175458PurposeProtein expression in bacterial cells. Entire CDS, M1-D1087.DepositorInsertEPB41L3 (EPB41L3 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB’
Plasmid#162703PurposepCB reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutation5513T>G (tetR E37A); 7758G>T (second tet op…PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Mal-B2
Plasmid#79756PurposeSingle fluorescent protein biosensor for maltoseDepositorInsertMal-B2
UseTags6xHisExpressionBacterialMutationPromoterpTrcAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcHELIX-sgRNA_entry (CJT94)
Plasmid#181783PurposeExpresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
A3RXB7
Plasmid#177694Purposeexpresses a N-acetyl hexosamine oxidase in E. coliDepositorInsertN-acetylhexosamine oxidase
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Alpha-Synuclein-His
Plasmid#186802PurposeCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.DepositorInsertAlpha-Synuclein-His (SNCA Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-N
Plasmid#108078PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorInsertCCDB (ccdB(letD) )
UseTagsStrepIII-HA-BirA*ExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.mCherry
Plasmid#112520PurposeProB driven mCherry expression with synTTDepositorInsertProB mCherry TT
UseTagsExpressionBacterialMutationPromoterProBAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(800-868)
Plasmid#141345PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).DepositorInsertGB1His6-TEV-ALIX(800-868)
UseTagsGB1-His6-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCri-18a
Plasmid#61326PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-BoNT/E
Plasmid#168020PurposeExpresses BoNT/E-LC(1-411)-6xHis in bacterial cellsDepositorInsertBoNT/E-LC(1-411)-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoHELIX-sgRNA_entry (BO3)
Plasmid#181784PurposeExpresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET20b_Pa-PirA
Plasmid#128956Purposeexpresses Pseudomonas aeruginosa PirA in E. coliDepositorInsertferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
UseTags6xHis and pelB leaderExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS413-GAL1-luc*(-SKL)
Plasmid#40234DepositorInsertluc*(-SKL)
UseTagsExpressionBacterialMutationPromoterGAL1Available sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only
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