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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBXCA3H

Plasmid

#47072

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBS hBAF155

Plasmid

#17876

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF155 (SMARCC1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pCAT217

Plasmid

#134885

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-Ava3858-3856

Plasmid

#45745

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3856

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pJLG029

Plasmid

#192980

Purpose

BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification

Depositor

Clay Fuqua

Article

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

pruR-3xFLAG

Use

Tags

3XFLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pH6-TEV-mCherry

Plasmid

#132430

Purpose

Expresses His6-tagged mCherry in E. coli using T7 promoter

Depositor

Mark Arbing

Insert

mCherry

Use

Tags

N-terminal His6 tag with TEV protease site

Expression

Bacterial

Mutation

Codon optimized

Promoter

T7

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a(cd226)

Plasmid

#177900

Purpose

Bacterial expression of his10-tagged human cd226 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

CD226 (aa 288-336 only) (CD226 Human)

Use

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_12E

Plasmid

#98654

Purpose

Expresses MBP-tagged phosphomimetic FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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ALDH18A1

Plasmid

#38947

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ALDH18A1 (ALDH18A1 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCAT205

Plasmid

#134882

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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GST-GIV-CT

Plasmid

#69863

Purpose

Bacterial expression of GST tagged GIV-CT

Depositor

Pradipta Ghosh

Article

Garcia-Marcos et al J Biol Chem. 2010 Apr 23;285(17):12765-7

Insert

GIV/Girdin (CCDC88A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C terminal amino acids 1623-1870 of human GIV

Promoter

TAC

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/mNeptune2-nat

Plasmid

#120337

Purpose

Bacterial mNeptune2 labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Aug. 31, 2019

Availability

Academic Institutions and Nonprofits only

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PRM-GFP

Plasmid

#40127

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

CI inducible sfGFP

Use

Synthetic Biology

Tags

LVA ssrA tag

Expression

Bacterial

Mutation

Promoter

pRM

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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BB1_P_12_asn_mbfA

Plasmid

#90289

Purpose

BB1 promoter mbfA gene of Aspergillus niger between FS1 and FS2

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mbfA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GRvT

Plasmid

#87363

Purpose

Expresses GRvT, a tandem heterodimer green and red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GRvT

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

AraBad

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSHDY-PL03-mVenus_tetR

Plasmid

#137663

Purpose

Conjugative vector derived from pSHDY. Constitutive expression of the repressor tetR, as well as aTc-inducible expression of mVenus.

Depositor

Ilka Axmann

Article

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Insert

PL03:mVenus

Use

Tags

Expression

Bacterial

Mutation

Promoter

PL03

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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TOPO mTagBFP2

Plasmid

#68413

Purpose

TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mTagBFP2

Use

Gmap

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Ikaros

Plasmid

#11261

Depositor

Lisa Steiner

Article

Willett et al Dev Dyn. 2001 Dec . 222(4):694-8.

Insert

ikaros (ikzf1 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pAJM.717

Plasmid

#108517

Purpose

PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTet*-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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