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Plasmid#119104PurposeBacterial expression of human phox homology (PX) domain, SNX25-PX (506-628)DepositorInsertSNX25-PX (506-628) (SNX25 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3d-E2-25K-C170S
Plasmid#18892DepositorInsertUbiquitin conjugating enzyme E2-25K (UBE2K Human, Most likely H. sapiens)
UseTagsExpressionBacterialMutationCysteine 170 is replaced with a Serine residue.PromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTKIP-neo
Plasmid#41064DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTW211b5
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRTagsExpressionBacterialMutationsee manuscriptPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2NT
Plasmid#62257Purposeexpression of A2NT sgRNA from the arabinose-inducible promoterDepositorInsertA2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP.E222
Plasmid#138248PurposeThree input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promotersDepositorInsertsaraC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL7-pCMV7.1
Plasmid#155025PurposeN-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vectorDepositorInsertCullin 7 (CUL7 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p70rg 15
Plasmid#17831DepositorInsertE. coli optimized red flourescent protein (RFPec), GFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBTK1010 (pSL25)
Plasmid#191005PurposeExpresses blue chromoprotein on a broad host range RP4 origin plasmidDepositorInsertblue chromoprotein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB5c-GFP
Plasmid#35353DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWLxR5
Plasmid#149490PurposeBroad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCSW21
Plasmid#86836Purposebacterial expression of anti-CRISPR protein AcrIIA4DepositorInsertacrIIA4
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T moesin 1-577
Plasmid#11637DepositorInsertmoesin (MSN Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-DAXX-His
Plasmid#175782PurposeDAXX protein for bacteria expression systemDepositorInsertdeath domain associated protein (DAXX Human)
UseTagsGST and TEVExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM7066
Plasmid#214004PurposePrpsM driven expression of ssrA-tagged mScarlet-I (LVA)DepositorInsertmScarlet-I-LVA
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:mid.11
Plasmid#191921PurposeEncodes a GFP design, sf:mid.11DepositorInsertsf:mid.11
UseTagsHis TagExpressionBacterialMutationQ69M Y145F T167I L220V V224IPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:mid.7
Plasmid#191933PurposeEncodes a GFP design, sf:mid.7DepositorInsertsf:mid.7
UseTagsHis TagExpressionBacterialMutationS72T Y145F V224IPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28 acid.6
Plasmid#191912PurposeEncodes a GFP design, acid.6DepositorInsertacid.6
UseTagsHis TagExpressionBacterialMutationQ69L S72A Y145M T167V H181LPromoterT7Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only