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pHflu4
Plasmid#203882PurposepHI1 with insertion of monomeric red fluorescent protein (mRFP)DepositorInsertmonomeric red fluorescent protein
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hTGFa
Plasmid#199236PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertTransforming growth factor alpha, partial (TGFA Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization Control
Plasmid#186553PurposeNegative Control mediating endosomal localizationDepositorInsert10R-8His-EGFP
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ShadowG-SUMO1
Plasmid#175239PurposeBacterial expression and purification, conjugatable SUMO for SUMOylationDepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
P22_wtCP_SP141his
Plasmid#122651PurposeExpression of P22 virus like particle in bacteriaDepositorInsertP22 Coat: WT / SP: 6xHis-SP141
UseTagsExpressionBacterialMutationtruncated SP, P120H in the capsid scaffold (see …PromoterAvailable sinceAug. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p-spTorA-GFP-H6C
Plasmid#168517PurposeGFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.DepositorInsertspTorA-GFP-H6C
UseTags6xHisC tag and spTorA (signal peptide of E. coli …ExpressionBacterialMutationThe mut3 GFP variant with 5 additional mutations …PromoteraraBADAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a-PcAad1
Plasmid#163039Purposeexpression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporiumDepositorInsertaryl-alcohol dehydrogenase
UseTagsGST and His6ExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-HALO
Plasmid#188943PurposeT7 expression vector for making his6- and HA3-tagged HALO proteinDepositorInserthis6-HA3-HALO
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN1717
Plasmid#74696PurposeRPU standard plasmidDepositorInsertInsulated J23101-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3036 GST-HPV-16 E6
Plasmid#10849DepositorInsertHPV16 E6 (E6 Human papillomavirus)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Loxodonta Africana
Plasmid#75351PurposeNt His-tag Loxodonta Africana 89-1368DepositorInsertSacsin Loxodonta Africana 89-1368
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSG287
Plasmid#115606Purposesurface-displayed Ag2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-WT-N’His
Plasmid#117701PurposeExpression of WT mouse SOD1 with N- terminal His tagDepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
BbZIP-cALFA
Plasmid#160498PurposeFor the protein expression of BbZIP with ALFA tag at C-terminus in E. coliDepositorInsertBbZIP
UseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS mNotch1-IC (DM#270)
Plasmid#15079DepositorInsertNotch1-IC (Notch1 Mouse)
UseTagsExpressionBacterialMutationK1750APromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET26b(+)-ATI_QconCAT_1
Plasmid#163957PurposeExression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.DepositorInsertATI QconCAT
UseTagspolyhistidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK635
Plasmid#72473PurposepET28a modified to contain TEV protease fusion tag instead of T7 tagDepositorTypeEmpty backboneUseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only
