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Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorInsertALFY (WDFY3 Human)
UseTags6xHis-GST-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBL090J
Plasmid#195045PurposecCadC-CCB3.5 under the control of PBADDepositorInsertcCadC-CCB3.5
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)
Plasmid#191635PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadR)-DadR
Plasmid#191636PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementationDepositorInsertDadR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZFYVE9C-c009
Plasmid#53618Purposebacterial expression plasmidDepositorInsertZFYVE9C
UseTagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE81L-KOFP7
Plasmid#160330PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.DepositorInsertKOFP7
UseTags6xHis tagExpressionBacterialMutationPromoterT5Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptac-sfGFP
Plasmid#139934PurposeIPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLacI-Ptac-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-UBE2S
Plasmid#66714PurposeExpresses UBE2S (1-196) in E. coliDepositorInserte2-epf (UBE2S )
UseTagsGST-3CExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-CRY1-PHR
Plasmid#26035DepositorInsertCRY1 (CRY1 Mustard Weed)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-FUSN-mut
Plasmid#200970Purposeinduction of phase-separated condensate mediated by FUSN-IDR mutantDepositorInsertFUSNmut
UseLentiviralTagsExpressionBacterial and MammalianMutation15 Tyr-to-Ser substitutionsPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAT593
Plasmid#68909PurposeNanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO PYL1 (36-211)
Plasmid#177843PurposeBacterial Expression of PYL1DepositorInsertPYR1-like 1 (PYL1 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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JF001A
Plasmid#53116Purposeexpresses alpha hemolysin under the control of theophylline riboswitchDepositorInserttheophylline riboswitch and alpha hemolysin
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMJ824
Plasmid#39314DepositorInsertCas9
UseCRISPRTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
6974 pPROEX-HTb-SSB1
Plasmid#1232DepositorInsertssb1 (SSB1 Budding Yeast)
UseTagshis tag (tev cleavable)ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pET28C-mCherry-DDX4LCD
Plasmid#204408PurposeE. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tagDepositorInsertDDX4(1-236) (DDX4 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-HotPETase
Plasmid#218701PurposeEncodes codon optimized HotPETase enzyme for bacterial expressionDepositorInsertHotPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
UseTags6xHis tag and HRV3C cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only