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Showing: 6121 - 6140 of 28446 results

  5. ATX3-77Q

    Plasmid
    #184249
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Codon optimization for protein expression in BL2…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184249-plasmid-map-sequence-id-370367

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  6. RGS16

    Plasmid
    #39140
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RGS16 (RGS16 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. 6xHis-MBP-huTsCas12a

    Plasmid
    #174687
    Purpose
    Expresses 6xHis-MBP tagged TsCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huTsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174687-plasmid-map-sequence-id-346204

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  8. pCB11

    Plasmid
    #201111
    Purpose
    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.
    Depositor
    Mary Dunlop
    Article
    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:
    Inserts
    CcaR
    tetA(C)
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C-to-T transition at pos. no. 1049 of tetA(C) lea…
    Promoter
    J23100 and PcpcG2-172
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    201111-plasmid-map-sequence-id-403029

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  10. pXW109Hg(RS-RinA-E11)2

    Plasmid
    #123151
    Purpose
    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, PmerT, PhrpLE, PrinA
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123151-plasmid-map-sequence-id-244015

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  13. pMSP1D1R1

    Plasmid
    #97323
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1R1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of R residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97323-plasmid-map-sequence-id-182662

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  15. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  18. pOGG026

    Plasmid
    #113992
    Purpose
    Level 1 golden gate cloning vector for low copy number stable environmental gene expression
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    113992-plasmid-map-sequence-id-369475

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  20. pMycoFos

    Plasmid
    #84577
    Purpose
    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.
    Depositor
    Nicholas Coleman
    Article
    Ly et al J Microbiol Methods. 2011 Sep;86(3):320-
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    84577-plasmid-map-sequence-id-175425

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Showing: 6121 - 6140 of 28446 results