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Plasmid#79740PurposeThis plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMARK3 (MARK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBb(B5)1k-GFPuv
Plasmid#106393PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1-B5 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIRCgp41-1
Plasmid#74227PurposeVector for SICLOPPS-mediated protein circularization using the gp41-1 intein.DepositorTypeEmpty backboneUseSynthetic BiologyTagsgp41-1 C-intein and gp41-1 N-inteinExpressionBacterialMutationPromoterT7-lacAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
SRpHi1
Plasmid#102541PurposeBacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-EYFP
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
HERC2 (3KCI)
Plasmid#25344PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertherc2.3951.4321 (HERC2 Human)
UseTagsHisExpressionBacterialMutationcontains amino acid residues 3951-4321PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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FGD5
Plasmid#28109PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertFGD5:HPC09X-H08:C212499 (FGD5 Human)
UseTagsHis-tag with TEV siteExpressionBacterialMutationcontains amino acid residues 889-1304.PromoterAvailable sinceMarch 21, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
APneg-SNAP25d
Plasmid#168014Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZH516
Plasmid#102669PurposeConstitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c012
Plasmid#173099PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP14 (PARP14 Human)
UseTagsmCeruleanExpressionBacterialMutationW1003-A1190PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-SNAP-KanMX6
Plasmid#87024PurposeTag S. pombe genes in C terminal with SNAPDepositorInsertSNAP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC-28S15-0X
Plasmid#20316DepositorInsertPsmyc-tetR#28
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEE077
Plasmid#176834PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert716
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
UseTags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK080
Plasmid#65187PurposeEncodes ZeocinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertZeocinR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pETCON-PTX-S1-220
Plasmid#67747PurposePertussis Toxin Subunit 1 Yeast Display PlasmidDepositorInsertPTxS1-220
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluA-NP
Plasmid#165092Purposeprotein expression in E. coliDepositorInsertInfluenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINO_H3
Plasmid#171927PurposeBacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_H3
UseTagsHis6ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits