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Plasmid#79644PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertJMJD1C (JMJD1C Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mKate2
Plasmid#199249PurposeFluorescent labelling of bacterial cells with mKate2DepositorInsertCodon-optimized mKate2 gene
UseTagsExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AWP-031
Plasmid#213967PurposeExpresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.DepositorInsertCas12a (cas12a Segatella bryantii, Synthetic)
UseTags3XFLAGExpressionBacterialMutationD875A to inactivate target nuclease activityPromoterPcfxA, IPTG inducibleAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATOH1_pUC18
Plasmid#191250PurposeEncodes the sequence for the ATOH1 gene (human)DepositorInsertATOH1 (ATOH1 Human)
UseTagsExpressionBacterialMutationPromoterlac promoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Skm1-PH
Plasmid#71371Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertSkm1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa1-132PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBY2_ligD(FnCpf1)
Plasmid#104623PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligDDepositorInsertcrRNA to ligD
UseCRISPRTagsExpressionBacterialMutationPromoterPj23119Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL-1
Plasmid#180422PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.DepositorInsertRed Chromprotein gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N02_switch
Plasmid#132742PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporterDepositorInsertToeRepG2_N02_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-Prha-mVenus_rhaS
Plasmid#137662PurposeConjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.DepositorInsertPrha:mVenus
UseTagsExpressionBacterialMutationrhaS (Arg218Leu)PromoterPrhaAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSG318
Plasmid#115595Purposesurface-displayed Ag2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pVRa20_992
Plasmid#49673DepositorInsertECF20_992
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP3
Plasmid#75456PurposePlasmid for constitutive expression of gfp-pdt3DepositorInsertgfp-pdt#3
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
Plasmid#55209DepositorTypeEmpty backboneUseTagsHis10 and MPB, Asn10ExpressionBacterialMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p15aC-4D1D-5
Plasmid#107866PurposeBacterial expression of PIS, PI4K and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β
UseTagsmycExpressionBacterialMutationPromoterproD and proD (in operon)Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
6C1A (MBD2)
Plasmid#119958PurposeBacterial expression for structure determinationDepositorInsertMBD2 (MBD2 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET FLII12Pglu-700uDelta6
Plasmid#13568DepositorInsertFLIPglu F16A Y12S-ECFP-D13R, 6aa linker
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBPromoterAvailable sinceFeb. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMLS7
Plasmid#32056PurposeVector optimized for constitutive gene expression in Burkholderia genus and related bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPs7 promoter, originated from Paraburkholderia xe…Available sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbA0c-RFP
Plasmid#35373DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbS0k-RFP
Plasmid#35369DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only