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Plasmid#188334PurposePCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mTagBFP2::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL004
Plasmid#180608PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertionDepositorInsertPlmCasX with DpbCasX R1 loop
UseCRISPRTags10xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK633
Plasmid#72397PurposeProduces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)DepositorInsertphosphoribosylaminoimidazole synthetase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK515
Plasmid#72385PurposeProduces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)DepositorInsertphosphoribosylglycinamide formyltransferase 2
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-TPX2(1-43)
Plasmid#69107PurposeFor bacterial expression of human TPX2(1-43aa) tagged with GST.DepositorInsertTargeting Protein for Xklp2 (TPX2 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 1-43 expressed.PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pANTO5
Plasmid#131106PurposeTo generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integraseDepositorInserttetR gene by pFRA61 (Boldrin et al 2010)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6-Twin-Strep-SUMO-pET28a-EfaCas2
Plasmid#112794PurposeTo express enterococcus faecalis Cas2 protein in E. coliDepositorInsertEfaCas2
UseTagsHis6-Twin-Strep-SUMOExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT K842M
Plasmid#154272PurposeBacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationLysine 842 changed to Methionine- bacterial codon…PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-Rac1-T17N
Plasmid#12978DepositorInsertRac1 DN (RAC1 Human)
UseTagsGSTExpressionBacterialMutationT17N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-merR_O
Plasmid#127023PurposeBasic Part O- ORF; merR; transcription factorDepositorInsertPart merR_o
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB092a
Plasmid#79217PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1+2); Ppro1Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pEB1-moxCerulean3
Plasmid#103975PurposePlasmid encoding moxCerulean3DepositorInsertmoxCerulean3
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-A4DARPin
Plasmid#198288PurposeProduces PVCs retargeted with anti-lysozyme DARPin A4DepositorInsertPVCpnf_pvc13-A4DARPin
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZ8-Prp
Plasmid#74065PurposepZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanRDepositorInsertPropionate inducible promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterprpAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPI1_pet28a
Plasmid#131653PurposeBacterial expression of ETS gene SPI1DepositorInsertSPI1 (SPI1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK1005 (pSL4)
Plasmid#191000PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_47
Plasmid#109245PurposepSC101 var5 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MAD2L1_HORMA
Plasmid#178480PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertMAD2L1_HORMA (MAD2L1 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJF076Sa
Plasmid#113322PurposeJ1-mRFP1DepositorInsertmRFP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only