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Showing: 5961 - 5980 of 27103 results

  3. pDual-eGFP(H66)

    Plasmid
    #63558
    Purpose
    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(H66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Availability
    Academic Institutions and Nonprofits only

  4. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Availability
    Academic Institutions and Nonprofits only

  8. pCol1a1-frt (FVB)

    Plasmid
    #63575
    Purpose
    Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Col1A-frt-hygro-pA
    Use
    Mouse Targeting; Frt/flpe
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
    63575-plasmid-map-sequence-id-105719

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  10. pET15b-ACP (from S. aureus)

    Plasmid
    #63686
    Purpose
    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus
    Depositor
    Michael Johnson
    Insert
    ACP
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  11. pET15b-ACPs (from S. pneumonia)

    Plasmid
    #63687
    Purpose
    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae
    Depositor
    Michael Johnson
    Insert
    acpS
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  13. pCVD15

    Plasmid
    #63689
    Purpose
    containing the bottom toxin fragment from a HindIII digest of V. cholerae 395 chromosome
    Depositor
    James Kaper
    Article
    Kaper et al Nature Biotechnology 2, 345 - 349 (1984)
    Insert
    bottom toxin fragment from a HindIII digest of V. cholerae 395 chromosome
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. pCVD30

    Plasmid
    #63691
    Purpose
    pCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.
    Depositor
    James Kaper
    Article
    Kaper et al Nature Biotechnology 2, 345 - 349 (1984)
    Insert
    V. cholera chromosomal DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    540 bp of CT A is deleted
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  16. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63722-plasmid-map-sequence-id-106345

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  17. pET28-iLOVf

    Plasmid
    #63723
    Purpose
    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63723-plasmid-map-sequence-id-110780

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Showing: 5961 - 5980 of 27103 results