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TOPO GFP
Plasmid#68488PurposeTOPO construct expressing GFP for generation of GMAP-compatible geneDepositorInsertGreen Fluorescent Protein
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSHDY
Plasmid#137661PurposeConjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB107a
Plasmid#79218PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW039
Plasmid#140035PurposeKO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercLac143Available sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTvL1-SGC
Plasmid#39188PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsGST TEVExpressionBacterialMutationPromoterTacAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
C1QBP
Plasmid#32863PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC1QBP (C1QBP Human)
UseTagsHisExpressionBacterialMutationamino acids E89-Q282 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJBL725
Plasmid#140395PurposeExpresses SmtB protein under the control of T7-lacO promoterDepositorInsertT7-lacO-SmtB-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP3 CKK
Plasmid#59033Purposeexpresses CKK domain of CAMSAP3 in E. coliDepositorInsertCAMSAP3 CKK domain (CAMSAP3 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-DsRed-Express2
Plasmid#111251PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2DepositorInsertDsRed-Express2
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIgnaviCas9
Plasmid#127595PurposeExpresses IgnaviCas9 in E. coliDepositorInsertIgnaviCas9
UseTags6xHis Tag and Maltose binding proteinExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAN-NOR
Plasmid#62306Purposesynthetic circuit: NOR gateDepositorInsertsA2NT sgRNA
A2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPPhlF and pBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-hRNF216
Plasmid#167354PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cellsDepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM30-C9ORF78_F8A
Plasmid#193369PurposeExpression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorInsertC9ORF78_F8A (C9orf78 Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationF8A, the mutation is located in the binding inter…PromoterT7Available sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2+Nterm Spe1 +Cterm Age1 site
Plasmid#88914PurposeFor expression of modified SpyCas9 in e.coli.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Map7-1333-end
Plasmid#46070DepositorInsertMap7-1333-end (Map7 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUG-natNT2
Plasmid#110922Purposeexpression of nat gene conferring resistance to nourseothricin for gene deletion in yeastDepositorInsertnat
UseTagsExpressionBacterial and YeastMutationPromoterAgTEF1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24b-BG1.3
Plasmid#199437Purposeexpresses BAmGreen1.3 in bacterial cellsDepositorInsertBAMGreen1.3
UseTagshis tagExpressionBacterialMutationPromoterT7Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only
