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Plasmid#186314PurposePlasmid for inducible E coli expression of the Z18 helixCAM.DepositorInsertZ18-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGM190oriT
Plasmid#115679PurposeE. coli-Streptomyces shuttle vector for gene expressionDepositorInsertoriT, aphII, tsr
UseTagsExpressionBacterialMutationPromotertipAAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
6CC8 (MBD3)
Plasmid#119959PurposeBacterial expression for structure determinationDepositorInsertMBD3 (MBD3 Human)
UseTagsGSTtag, 6xHis and ThrombinExpressionBacterialMutationMBD domain (contains residues 1-71)PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-NTOM20-mVenus-Zdk3
Plasmid#81012PurposeExpression of Zdk1 anchored to mitochondriaDepositorInsertZdk3
UseTags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb720-HO
Plasmid#141201PurposeExpresses mRhubarb720 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb720
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, L196Q, T202D, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w2-Prd
Plasmid#18038DepositorInsertPaired (prd Fly)
UseTagsExpressionBacterialMutationPaired is simply used as an insert between Kpn1 a…PromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-link-CGFP
Plasmid#52733PurposeL-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assayDepositorInsertlink-CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
rpoS200-RFP
Plasmid#63840PurposeThis plasmid is designed as a bacterial stationary phase sensorDepositorInsertrpoS, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is translationally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC71173
Plasmid#154061PurposeLevel 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-CreDepositorInsertCre recombinase
UseTagsExpressionBacterialMutationIntroduction of U5 intron sequences, to prevent p…PromoterHvHSP17Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p7XNH3
Plasmid#47064PurposeFX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRaGE Pyl TAG GFP Y35TAG
Plasmid#160041PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.DepositorInsertsPyrrolysyl tRNA synthetase (Methanosarcina mazei)
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
deGFP
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationChanged tyrosine 35 to TAG stop-codon (Site numbe…PromoterP70b promoter and T500 terminator, Vibrio natrieg…Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-CT (CamR, P21)
Plasmid#45981DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHG4
Plasmid#188252PurposeIntegrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoteract1p/actII-orf4Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE165
Plasmid#118513Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSP32
Plasmid#199374PurposeEncodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTPH418b
Plasmid#185725PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only