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Plasmid#140755PurposeFRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate RatioDepositorInsertLapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTST6
Plasmid#86603PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.DepositorInsertMTST6
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046760
Plasmid#145600PurposeBacterial Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-DONSON
Plasmid#208797PurposeXenopus laevis His6-Myc-DONSON wild typeDepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAB088h
Plasmid#79215PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-TnCAD-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1+2); Ppro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScaf-1512.1
Plasmid#111402PurposepScaf phagemid with insert for generating DNA origami scaffold 1512 bases in lengthDepositorInsertpScaf-1512
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH-MBP
Plasmid#101768PurposeGateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion proteinDepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dead Streptavidin-SpyTag
Plasmid#59548PurposepET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramersDepositorInsertDead Streptavidin-SpyTag
UseTagsSpyTagExpressionBacterialMutationThe construct lacks final K of SpyTagPromoterT7Available sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2/SVNeo
Plasmid#26553DepositorInsertpT2/SVNeo
UseTagsExpressionBacterialMutationadded the Rmut(80, 87, d101)PromoterAvailable sinceMarch 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3 I0500 LasI
Plasmid#107966PurposeArabinose-inducible 3OC12HSL sender plasmidDepositorInsertlasI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTetQCas-BsaI
Plasmid#170629PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1184
Plasmid#205965PurposeNZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)DepositorInsertDinG HNH
UseTags14xHis-TwinStrep-bdSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -