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Plasmid#11944DepositorInsert14-3-3 sigma (SFN Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCasX1
Plasmid#87685Purposep15A plasmid expressing CasX locus from DeltaproteobacteriaDepositorInsertCasX1
UseTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ20
Plasmid#65027PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneUseTagslacZExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-(SIM)1
Plasmid#126954PurposeBacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagseGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6c3
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJWV25
Plasmid#61045PurposeTo construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaADepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-SP44s-GUS
Plasmid#172430PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidaseDepositorInsertbeta-D-glucuronidase
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterSP44sAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ETh_rhi483
Plasmid#180429PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ETh_rhi483
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2B-56
Plasmid#177925PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
UseTagsExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterMYO2Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-T20S
Plasmid#110805Purposeexpresses Thermoplasma acidophilium 20S proteasome in E. ColiDepositorInsertsPsmB
PsmA
UseTagstev-HisExpressionBacterialMutationN2D and Q2EPromoterT7 and T7 promoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV125
Plasmid#63191PurposepRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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A-BRDA4-3
Plasmid#166513PurposescFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 333-460 of 1362, Bromodomain 2DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLC291
Plasmid#44448DepositorTypeEmpty backboneUseRk2, pmb1TagsExpressionBacterialMutationPromoterP_R (Phage 16-3)Available sinceAug. 30, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pExp-NHis
Plasmid#112558PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-EGFP
Plasmid#182641PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
UseTagsBAP, EGFP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KT (KanR, P21)
Plasmid#45987DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-pro-IL1B
Plasmid#183386PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL1B (Il1b Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only