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Plasmid#180605PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasXDepositorInsertDpbCasX
UseCRISPRTags10x His and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-DmPABPC1_J
Plasmid#146647PurposeBacterial Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionBacterialMutationG26S relative to NM_166265.2/NP_725750.1PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR
Plasmid#47643Purposewt esaR gene under control of Plac in pACYC184DepositorInsertPlac-esaR-terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGT-B1
Plasmid#122579PurposeMAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT1
Plasmid#53557Purposebacterial expression of IFIT1DepositorInsertIFIT1 (IFIT1 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEQ111m
Plasmid#40190DepositorInsertEcoRI endonuclease & EcoRI methylase
UseTagsExpressionBacterialMutationchanged Glutamic Acid 111 to GlutaminePromoterLambda PLAvailable sinceNov. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_WT
Plasmid#98657PurposeExpresses 6xHis-tagged hnRNPA2 AA 190-341DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)
Plasmid#159689PurposeE. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)DepositorInsertHis6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMCSG28-SH3-588
Plasmid#185920PurposeExpresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferaseDepositorInsertSH3-588
UseTags6x HIS tagExpressionBacterialMutationDesigned enzyme with novel interface between huma…PromoterT7Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p40R57Q PX
Plasmid#19009DepositorInsertp40phox (NCF4 Human)
UseTagsGSTExpressionBacterialMutationaa1-148 (PX domains) site directed mutagenesis: …PromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (p15A origin)
Plasmid#74090PurposepTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterR0010 Const. Promoter controlling RFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAT203
Plasmid#134880PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
UseTags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pHYRSF1
Plasmid#34549DepositorInsertDnaE N-intein
UseTagsH6-GB1ExpressionBacterialMutationPromoterT7Available sinceJan. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXint129
Plasmid#178208PurposeEncodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.DepositorUseTagsExpressionBacterialMutationPromoterlacIAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR11 WT NFAT1
Plasmid#11791DepositorInsertNFAT1 (Nfatc2 Mouse)
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn G51D
Plasmid#105747PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein G51D (SNCA Human)
UseTagsExpressionBacterialMutationG51DPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only