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Plasmid#64025PurposePlasmid for integration into neutral site of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only
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cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_vsfGFP9
Plasmid#191747PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and vsfGFP-9ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-PRM-B0034-geneVI
Plasmid#80858PurposeAccessory plasmid (encodes M13 gene VI)DepositorInsertM13 gene VI
UseTagsExpressionBacterialMutationPromoterLambda PrmAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTrcHUM
Plasmid#19003DepositorInsertgamma-humulene synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-pBAD
Plasmid#54220PurposeLocalization: Protein Expression Vector , Excitation: 487, Emission: 509DepositorTypeEmpty backboneUseTags6xHis and mEmeraldExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNatA (pACYCduet-naa10-naa15)
Plasmid#72928PurposeAllows expression of a modified version of the fission yeast NatA complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationCodon optomised for E.coli expressionPromoterT7Available sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-cpLuc2
Plasmid#135629PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)DepositorInsertNAD-cpLuc2 sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMD18-T-pyrF
Plasmid#174388Purposemodified pMD18-T containing pyrF and its native promoter, AmprDepositorInsertpyrF
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
Plasmid#176534PurposeBacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrepDepositorInsertCRISPR-associated IscB (Delaware Bay metagenome)
UseTags14xHis-MBP-bdSUMO and TwinStrepExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC059-HisMS2_PLP_NucPhos_pac
Plasmid#155039PurposeGeneration of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein geneDepositorInsertsMaturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein
UseTagsExpressionBacterialMutationRemove TypeIIs Restriction SitesPromoterT7Available sinceSept. 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAP05
Plasmid#186261PurposesfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoterDepositorInsertsUseTagsExpressionBacterialMutationPromoterBBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)
Plasmid#169193PurposeTo express SARS-CoV-2 Papain-like protease in E. coliDepositorInsert14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
UseTags14His-SUMOExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP3-Halo
Plasmid#175310PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP3 (IGF2BP3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS102
Plasmid#118281PurposeExpresses GFPmut2 with low cell-to-cell variation and lactose inductionDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterpLlacO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1 U1-C-FL
Plasmid#129077Purposegeneration of GST-tagged U1-C protein using bacteriaDepositorInsertU1-C (SNRPC Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -