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Plasmid#111493Purposetranslational initiation factor 1DepositorInserttranslational initiation factor 1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Omega 1
Plasmid#118046PurposeGoldenBraid2.0 Omega 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1
Plasmid#122083PurposeExpress M.EcoGII fused to LaminB1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-LaminB1
UseRetroviralTagsV5 tag on Nter of LaminB1ExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
poRibo-T2
Plasmid#69347PurposeRibo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutationDepositorInsertoRibo-T
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pramr-GFP
Plasmid#194148PurposeContains a RamR-regulated promoter expressing GFPDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterPramrAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTCF200 Mouse beta-2 microglobulin
Plasmid#180462PurposeE. coli expression of class I MHC light chainDepositorInsertMouse beta-2 microglobulin
UseTagsBSP41ExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWT004a
Plasmid#107883PurposeR1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C
Plasmid#122085PurposeExpress M.EcoGII fused to CENPC with destabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
UseRetroviralTagsV5 tag on Nter of CENPCExpressionBacterial and MammalianMutationL341F, R930L (see comments below)PromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PARP9-c020
Plasmid#173097PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP9 (PARP9 Human)
UseTagsmCeruleanExpressionBacterialMutationN310-N493PromoterAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotertac promoter from pKK233-2Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-3C-EmPNGaseF
Plasmid#218193PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseFDepositorInsertPNGaseF
UseTagstufA-His-CBD-3CExpressionBacterialMutationcodon optimized Ecoli, residues 41-354PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL1203
Plasmid#196024PurposeExpression/purification of 6xHis-TEV-MlaCDepositorInsertMlaC
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D
Plasmid#159571PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D (KRAS Human)
UseTagsHis6-MBP-tev-AviExpressionBacterialMutationG13DPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pECTO
Plasmid#67683PurposepMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureusDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLC-255
Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AsCpf1-2NLS
Plasmid#102565Purposebacterial expression of AsCpf1-2NLSDepositorInsertAsCpf1
UseTags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
UseTagsHA-6HIS and MKIEEExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQFT
Plasmid#203674PurposepQF derivative with promoter PQ5 exchanged for promoter PQJDepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only