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Showing: 5401 - 5420 of 29407 results

  2. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid
    #108693
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme
    Depositor
    Martin Reijns, Andrew Jackson
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon, …
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108693-plasmid-map-sequence-id-208987

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  3. pKI1

    Plasmid
    #199118
    Purpose
    Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling
    Depositor
    Jeff Gelles
    Article
    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12
    Insert
    H6-SNAP-RapA
    Use
    Tags
    6xHis and SNAP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199118-plasmid-map-sequence-id-388190

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  6. pJA48 HLA-B*57:01

    Plasmid
    #180444
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*57:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    180444-plasmid-map-sequence-id-366471

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  7. Mut2-RS

    Plasmid
    #182882
    Purpose
    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
    Depositor
    Miriam Amiram
    Article
    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384
    Insert
    Mut2-RS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    182882-plasmid-map-sequence-id-366061

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  11. As4

    Plasmid
    #123161
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J115-30arsR-t-ParsR-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J115-30arsR-t-ParsR-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J115, ParsR
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123161-plasmid-map-sequence-id-243984

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  13. proinsulin-msGFP2 (C48S C70S)

    Plasmid
    #218956
    Purpose
    Expresses proinsulin fused to msGFP2 (C48S C70S) in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-msGFP2 C48S C70S
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218956-plasmid-map-sequence-id-446162

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  18. YWHAG

    Plasmid
    #36903
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YWHAG (YWHAG Human)
    Use
    Tags
    6xHis with TEV Cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. CYP51A1 (3I3K)

    Plasmid
    #25161
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP51A1_cw_09 (CYP51A1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 54-502
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5401 - 5420 of 29407 results