Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 521 - 540 of 27067 results

  1. SOCS2A-c016

    Plasmid
    #53689
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    SOCS2A
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;ā€¦
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  2. pACĪ»CI

    Plasmid
    #53730
    Purpose
    PlacUV5-directed synthesis of the Ī»CI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    bacteriophage lambda CI protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac UV5
    Availability
    Academic Institutions and Nonprofits only
    53730-plasmid-map-sequence-id-83908

    Enlarge

  3. pBRĪ±

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    Ī±NTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

    Enlarge

  4. pBRĪ±-Ļƒ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Ļƒ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Availability
    Academic Institutions and Nonprofits only

  10. pET21a-LIC

    Plasmid
    #62303
    Purpose
    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    62303-plasmid-map-sequence-id-102191

    Enlarge

  15. pET32a-LIC

    Plasmid
    #62310
    Purpose
    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis, S-tag, and Trx-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
    62310-plasmid-map-sequence-id-326570

    Enlarge

  16. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only

  17. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV proteā€¦
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

    Enlarge

  18. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter from pKK233-2
    Availability
    Academic Institutions and Nonprofits only
    62343-plasmid-map-sequence-id-102266

    Enlarge

  19. p28BIOH-LIC

    Plasmid
    #62352
    Purpose
    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
    62352-plasmid-map-sequence-id-102272

    Enlarge

  20. PLEXIN A2 (m1)

    Plasmid
    #62353
    Purpose
    Riboprobe for in vitro transcription of Plexin A2 (m1)
    Depositor
    Christiana Ruhrberg
    Insert
    Plexin A2 (Plxna2 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    bp 2430-3064 of Genbank ref NM_008882.2
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 521 - 540 of 27067 results