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Plasmid#61262PurposeSucrose counterselection (sacB) vector for unmarked allelic exchange.DepositorTypeEmpty backboneUseSynthetic Biology; Sucrose counterselectionTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only
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pE-SUMO CRYAA
Plasmid#80756PurposeExpression of human alpha A-crystallin in E. coliDepositorInsertCRYAA (CRYAA Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
Plasmid#135454PurposeBacterial expression of TEVΔ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability. Truncated form…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrcAgBIS (CO)
Plasmid#35153DepositorInsertBisabolene Synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpTrcAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
B1-4
Plasmid#111466Purposetranslational elongation factor TuDepositorInserttranslational elongation factor Tu
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBL7045
Plasmid#167216Purposeoverexpression of CueR in E. coli for cell-free copper sensingDepositorInsertCueR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
DT049A
Plasmid#159410PurposeConstitutive murine mature BDNF expression, controlled under E.coli tet promoterDepositorInsertpr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromotertet promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MP5
Plasmid#69677PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT6
Plasmid#34700DepositorInsertPMRT6
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
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p2CT-His-MBP-Lse_C2c2_WT
Plasmid#83486PurposeBacterial expression of WT L. seeligeri C2c2 CRISPR effectorDepositorInsertLse_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOGG005
Plasmid#113980PurposeLevel 1 high copy number vector for E. coli expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDBamHI-Leu
Plasmid#38256DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
371-GFP
Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneUseTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pACYC-GST
Plasmid#62329PurposeE. coli pACYC based expression vector with N-terminal TEV cleavable GST-tagDepositorTypeEmpty backboneUseTagsGlutathione S-transferase (GST) tag and TEV prote…ExpressionBacterialMutationPromoterT7-lac0Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CJ8421_04975 Rptr2 and CJ8421_04975 mRNA
Plasmid#194210PurposeExpresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2DepositorInsertCJ8421_04975 mRNA and CJ8421_04975 Rptr2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcMscL
Plasmid#92418PurposeE. coli Mechanosensitive Channel of Large ConductanceDepositorInsertMscL (mscL E. coli)
UseTags6x Histidine Tag and Thrombin Cleavage Site betwe…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC018 - LshCas13a from Leptotrichia shahii
Plasmid#91908PurposeExpresses LshCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLshCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNASE1_Exo-endo-phos
Plasmid#110004PurposeProtein expression and purification of DNASE1_Exo-endo-phosDepositorInsertDNASE1_Exo-endo-phos (DNASE1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only