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Plasmid#145685PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
BB1_12_YlpGPD
Plasmid#117820PurposePromoter pGPD of Y. lipolytica for GoldenMOCSDepositorInsertYlpGPD
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-152
Plasmid#118534PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
R619-X67-527: His6-SARS-CoV-2 N
Plasmid#170204PurposeE. coli expression of SARS-CoV-2 nucleocapsid proteinDepositorInsertSARS-CoV-2 N (N SARS-CoV-2)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F
Plasmid#39264DepositorTypeEmpty backboneUseBacterial expression, bacterial expression of re…Tags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCJ191
Plasmid#162668PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 884-1066-ECFP FRET
Plasmid#46179DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP, EYFP, and HisExpressionBacterialMutationaa 884-1066 (Vt, tail domain)PromoterT7Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1_6xHis-TEV-GABARAP-Gly
Plasmid#190930PurposePlasmid for the expression and purification of GABARAP. Internal Reference: SMC894DepositorInsertGABARAP (GABARAP Human)
UseTags6xHis-TEVExpressionBacterialMutationEnds with Glycine 116PromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
19 pCOLI_A_SUMO
Plasmid#160257PurposeN-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(WT)
Plasmid#111323Purposefor T7 transcription of wt actin pre-mRNADepositorInsertACT1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET28CLY5
Plasmid#21764DepositorInsertECFP -EYFP linked by flexible linker containing 5 SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
MS2-Snapf
Plasmid#65860PurposeExpresses RNA-binding protein MS2 with fast SNAP fusion tagDepositorInsertsMS2
Fast SNAP
UseTagsHA and NLSExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDB180
Plasmid#80677Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged proteinDepositorTypeEmpty backboneUseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR47S Cm
Plasmid#41892DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacbTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorInsertLuxI
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
NpuDnaEc-cGCaMP2
Plasmid#34851DepositorInsertNpuDnaEc-cGCaMP2
UseTagsExpressionBacterial and MammalianMutationPromoterCMV, T7 lacAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCM271rfp
Plasmid#59774PurposepCMrfp with TrfA mutation (271R to C)DepositorInsertRFP
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-147
Plasmid#118563PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only