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Plasmid#119104PurposeBacterial expression of human phox homology (PX) domain, SNX25-PX (506-628)DepositorInsertSNX25-PX (506-628) (SNX25 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ITCH
Plasmid#36197PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH (ITCH Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains HECT domainPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSFDuet1-Cdc45
Plasmid#126878PurposeExpresses human Cdc45 in bacteriaDepositorInsertCDC45 (CDC45 Human)
UseTagsNo tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pTU2-A-RFP (p15A origin)
Plasmid#74090PurposepTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterR0010 Const. Promoter controlling RFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-NHis
Plasmid#112558PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET42a-lambdaN+-L+-GSH
Plasmid#98894PurposeExpresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo methodDepositorInsertlambdaN+-L+
UseTagsGST tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTPH418b
Plasmid#185725PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_266-341
Plasmid#118821PurposeBacterial expression of hnRNPA2 truncationDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHis and TEVExpressionBacterialMutationcontains amino acids 266-341PromoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSG312
Plasmid#115613Purposesurface-displayed Nb2, Arabinose-inducible; medium-copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)1
Plasmid#127089PurposeBacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP human SUMO3 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkHR113A
Plasmid#182292Purposeexpresses c-di-GMP mutant biosensor in E. coliDepositorInsertmVenusNB-MrkHR113A
UseTagsmVenusNB internal fusion to MrkH with a R113A poi…ExpressionBacterialMutationchanged arginine 113 to alanine, MrkH numbering …PromoterproCAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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C1QBP
Plasmid#32863PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC1QBP (C1QBP Human)
UseTagsHisExpressionBacterialMutationamino acids E89-Q282 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET16b-Rnl2(1-249)K227Q
Plasmid#14072DepositorInsertRnl2(1-249)
UseTagsHis (10x)ExpressionBacterialMutationchanged Lys227 to Glutamine Gly112 is actually C…PromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET26b(+)-ATI_QconCAT_1
Plasmid#163957PurposeExression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.DepositorInsertATI QconCAT
UseTagspolyhistidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM09
Plasmid#135482PurposeAgrobacterium T-DNA with SpunHsp70pr driving hph-GFPDepositorInsertSpunHsp70pr hph-GFP ADH1t
UseTagsGFPExpressionBacterial and YeastMutationPromoterSpunHsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK1A (DYRK1A Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -