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Plasmid#135191PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-CreI.DepositorInsertI-CreI lamba Red
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoteraraBADAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2-Rheb
Plasmid#15889DepositorInsertRheb (RHEB Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_09
Plasmid#194670PurposeProtein production of the split HaloTag based calcium recorder Caprola_09 in bacteriaDepositorInsertCaprola_09
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean
Plasmid#103968PurposePlasmid encoding mCeruleanDepositorInsertmCerulean
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterProCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAD16_CAD_12-490_pvp008
Plasmid#124842PurposeExpresses Aspergillus terreus CAD in E.coliDepositorInsertcis-aconitate decarboxylase
UseTagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-11, synthetic codon optimiz…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB045a
Plasmid#79224PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PHGDH
Plasmid#74113PurposeExpression of N-terminal His-taggedDepositorInsertPHGDH (PHGDH Human)
UseTagsHis-taggedExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-130
Plasmid#118516PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCS2-mkgDUX4
Plasmid#21156DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPS22 (pET23a-Cas10/Csm/csm2H6N)
Plasmid#70038PurposeBacterial expression of the S. epidermidis Cas10·Csm complexDepositorInsertCas10/Csm complex
UseTags6xHIS on N terminus of Csm2ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-Rac1-Q61L
Plasmid#12979DepositorInsertRac1 constitutively active (RAC1 Human)
UseTagsGSTExpressionBacterialMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRH2502
Plasmid#84379PurposeExpression of dcas9 D10A H840A from a TetR-regulated uvtetO promoterDepositorInsertdcas9
UseCRISPRTagsExpressionBacterialMutationAspartate at position 10 changed to Alanine, Hist…Promoteruv15tetOAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/mClover/nat
Plasmid#120330PurposeBacterial mClover labelingDepositorInsertmClover
UseTagsD103N (see comment below)ExpressionBacterialMutationPromoter(None)Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-MBP-TEV-GG
Plasmid#215794PurposeCloning of target gene with N terminal MBP tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHflu2
Plasmid#203880PurposepHI1 with insertion of ampicillin-resistance geneDepositorInsertbroad-spectrum class A beta-lactamase TEM-116
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mTurquoise2
Plasmid#108235PurposeBroad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPc promoter - mTurquoise2
UseTagsExpressionBacterialMutationcodon optimized for experession in P. fluorescensPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pExp-PpiB
Plasmid#112572PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-PpiB and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only