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pSB1C3-H3

Plasmid

#109381

Purpose

Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-AGO3

Plasmid

#24318

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

AGO3 (AGO3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pNR188

Plasmid

#79543

Purpose

Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig1

Plasmid

#64841

Purpose

A lentiviral vector for the expression of mouse Olig1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig1 (Olig1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGT-Ah1

Plasmid

#122568

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMYO127TAG-SepT

Plasmid

#34625

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

Myoglobin-127TAG

Use

Tags

6xHIS

Expression

Bacterial

Mutation

codon-optimized + an amber stop codon was introdu…

Promoter

Ipp

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NAD-Snifit

Plasmid

#113658

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_RRM1

Plasmid

#157865

Purpose

express His tagged hnRNPA2 RRM1

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pPROK AdoHcyase

Plasmid

#14683

Depositor

Michael Hershfield

Article

Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

Insert

adenosyl-homocysteine hydrolase (AHCY Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2007

Availability

Academic Institutions and Nonprofits only
human p53-(1-360)

Plasmid

#24865

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-360.

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

Plasmid

#110547

Purpose

CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.

Depositor

Ichiro Matsumura

Insert

dCas9

Use

Tags

Expression

Bacterial

Mutation

D10A, H840A

Promoter

pBAD

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGTM_COV2_NSP5_004_SUMO

Plasmid

#190062

Purpose

Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

Depositor

Gaetano Montelione

Article

Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

Insert

3CLpro (ORF1ab SARS-CoV-2)

Use

Tags

6X-His-Sumo

Expression

Bacterial

Mutation

Promoter

T7 lac

Available since

June 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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ABL1_HUMAN_D0

Plasmid

#79727

Purpose

This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

ABL1 (ABL1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

Plasmid

#191647

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Use

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xHA

Plasmid

#129567

Purpose

Empty backbone for HA tag

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET24a MDH_AQUAR

Plasmid

#204239

Purpose

Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDH_AQUAR

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-DARPinOFF7

Plasmid

#182398

Purpose

Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-DARPinOFF7

Use

Tags

Vesicle Nucleating peptide 6 (VNp6)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pNCS-mCardinal2

Plasmid

#52631

Purpose

bacterial expression vector with far-red FP mCardinal2

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mCardinal2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBT137-splitD

Plasmid

#122606

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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