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Plasmid#83313PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iL
UseTags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iL betwe…PromoterT5Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-Hsp90 C(626-732)
Plasmid#22483DepositorInsertHsp90alpha C domain (HSP90AA2P Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOVSD
Plasmid#81033PurposeMammalian expression of the super-dark state of LOV2DepositorInsertLOVSD
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450A, L514K, G528A, L531E, N538EPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
UseTagsGSGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-KanMX6
Plasmid#15983DepositorTypeEmpty backboneUseTags5xFlagExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
UseTags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST-KT
Plasmid#44561Purposefor ectopic inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-rsTagRFP
Plasmid#31927DepositorInsertrsTagRFP
UseTagsHisExpressionBacterialMutationF84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…PromoteraraBAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-ecDHFR (WT)
Plasmid#73188Purposebacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)DepositorInsertfolA dihydrofolate reductase (folA E. coli)
UseTagsHis 6XExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTrcAgBIS (CO)
Plasmid#35153DepositorInsertBisabolene Synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpTrcAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLJSRSF7
Plasmid#64693PurposeHis-MBP-Smt3-NpuDnaEN102-CBDDepositorTypeEmpty backboneUseTagsCBD, HisTag, MBP, NpuDnaE-N102, and Smt3ExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-BR1
Plasmid#160333PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.DepositorInsertBR1
UseTags6xHis tagExpressionBacterialMutationPromoterT5Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH474_pTech_Para-supP
Plasmid#163718PurposeArabinose-inducible expression of supPDepositorInsertsupP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
UseTags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pBAD/HisD-LSSmKate1
Plasmid#31908DepositorInsertLSSmKate1
UseTagsHISExpressionBacterialMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pD2_miniluc_newP7
Plasmid#174105Purposeadding the mini luciferase and mini promoter to the MPRA lenti constructDepositorInsertmini luciferase mini-promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-LgBiT
Plasmid#216779PurposeExpresses LgBiT in bacteriaDepositorInsertLgBiT
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS140_6xHis-EGFP-I3-01
Plasmid#211889PurposeBacterial expression of I3-01DepositorInsertI3-01
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only