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Showing: 4701 - 4720 of 28150 results

  10. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  12. pGEX-5X-1 U1-C-FL

    Plasmid
    #129077
    Purpose
    generation of GST-tagged U1-C protein using bacteria
    Depositor
    Takeshi Tsubata
    Insert
    U1-C (SNRPC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    129077-plasmid-map-sequence-id-252125

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  14. pETSG

    Plasmid
    #159418
    Purpose
    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli
    Depositor
    Dianfan Li
    Article
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Use
    Tags
    polyhistidine (poly-His)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159418-plasmid-map-sequence-id-321533

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  16. pUC19-T7-SdB-T

    Plasmid
    #87307
    Purpose
    Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Stabilized dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87307-plasmid-map-sequence-id-174665

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  18. pEXP5-NT/pT7 LuxI

    Plasmid
    #193626
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxI
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193626-plasmid-map-sequence-id-382174

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Showing: 4701 - 4720 of 28150 results