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Plasmid#98919PurposeConstitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.DepositorInsertPROPS
UseTagsHisExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)- PC-MjMAT
Plasmid#179372PurposePC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.DepositorInsertPC-MjMAT
UseTags6xHisExpressionBacterialMutationL147A, I351APromoterT7Available sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPCK
Plasmid#133128Purposea recombinant plasmid, expressing pckA PEP-carboxykinase) geneDepositorInsertPEP-carboxykinase gene
UseTagsExpressionBacterialMutationPromoterPEP-carboxykinase promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HS-GFP-PACT
Plasmid#105954Purposeheat shock inducible transgenesis, PACT is a centriol markerDepositorInsertPACT (AKAP9 Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP670nano3
Plasmid#184663PurposeMonomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmidDepositorInsertmiRFP670nano3
UseTagsHisExpressionBacterialMutationPromoteraraCAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWT021a
Plasmid#107895PurposeW2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0DepositorInsertPTetO-SD8-BE2, PLac-sgRNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-10xCTD
Plasmid#138472PurposeExpresses GST-tagged 10xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation10x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα13-LgB106
Plasmid#134364PurposeNanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.DepositorInsertGα13-LgB106 (GNA13 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 pET15b
Plasmid#60678PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSA-C
Plasmid#17329DepositorInsertstreptavidin
UseTagsExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…PromoterAvailable sinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBA620
Plasmid#186240PurposeLbuCas13a Negative Control (RFP targeting)DepositorInsertpTet-eLbuCas13a + crRNA negative control
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SARS-CoV-2 NSP13
Plasmid#176492PurposeExpresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.DepositorInsertSARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
UseTagsHis6 and T7ExpressionBacterialMutationI570 from SARS-CoV NSP13 was mutated to V to conv…PromoterT7Available sinceJan. 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETDest
Plasmid#124245PurposeAllows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTK077
Plasmid#65184PurposeEncodes KanamycinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertKanamycinR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-INSyibp-Nef
Plasmid#134593PurposeLow Phosphate inducible NefDepositorInsertHIV Nef protein
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAK464
Plasmid#140702PurposeNBαEGFPDepositorInsertNBαEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE130
Plasmid#118484Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11b-SpyCatcher-HA2
Plasmid#165991PurposeSpyCatcher-fused influenza HA ministem with removable HisTagDepositorInsertSpyCatcher-HA2
UseTagsFoldon, HisTag, and SpyCatcherExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TnY
Plasmid#163975PurposeIPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.DepositorInsertTnY
UseTagsMxe intein - Chitin-binding domainExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only