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Plasmid#62810PurposepPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1950
Plasmid#205983PurposeGa0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DRDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array
UseTags14xHis and TwinStrep-tagExpressionBacterialMutationPromoterT7 (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-WWP2
Plasmid#186807PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.DepositorInsertHis-Tev-WWP2 (WWP2 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFAK2 (PTK2B Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLQ-Pxyl/tet-cas9-Pj23119-sgRNA
Plasmid#92121PurposeCRISPR-Cas9 based efficient genome editing in S. aureusDepositorInsertCas9-Pxyltet-sgRNA-pj23119
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-truncated
Plasmid#198286PurposeContains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCsDepositorInsertPVCpnf_pvc13-truncated
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Mocr TEV LIC cloning vector (2O-T)
Plasmid#29710DepositorTypeEmpty backboneUseTagsHis6-Mocr-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-28GG
Plasmid#104655PurposeLevel 2 cloning vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
UseTagsExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBG100-TCPTP
Plasmid#33365DepositorInsertTCPTP-45 (PTPN2 Human)
UseTags6xHIS - cleavableExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-LPETGG
Plasmid#91697PurposeCohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGGDepositorInsertCohIII-CBM-HIS-LPETGG
UseTagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP
Plasmid#185013PurposeBacterial Expression of 6xHis Sirius-GFP Fusion ProteinDepositorInsertSirius-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT6
Plasmid#34700DepositorInsertPMRT6
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNO41
Plasmid#101067PurposeBphP1-PpsR2 chromophore plasmid; ho1DepositorInsertho1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
FL MALT1 WT in pET29b-Strep
Plasmid#48968PurposeExpresses Full length wild type MALT1 in e.coliDepositorInsertMALT1 isoform A (MALT1 Human)
UseTagsHis and StrepExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTHMT_hnRNPF_FL
Plasmid#139114Purposeexpress FL hnRNPF with N-terminal maltose binding protein tagDepositorInserthnRNPF (HNRNPF Human)
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
XXBLM (XXBLMA-c075)
Plasmid#98223PurposeVector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tagDepositorInsertXXBLMA (BLM Human)
UseTagsHis-6ExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_49
Plasmid#109247PurposepSC101 var7 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only