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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Use

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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(SUMO)10-(SIM)5

Plasmid

#126866

Purpose

Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

Plasmid

#185713

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 and F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss, Tadhg Begley

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Use

Tags

6xHis-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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PB-TRE3G-SOX17

Plasmid

#104541

Purpose

PiggyBac vector encoding doxycycline-inducible human SOX17

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

SRY-box 17 (SOX17 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3G

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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katushka-FMDV-P2A-pTRIP

Plasmid

#163522

Purpose

Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

Depositor

Thomas Weber

Insert

stopless Katushka-P2A

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAG247 His-iFAST

Plasmid

#130808

Purpose

Expresses His-tagged iFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pIV pm

Plasmid

#218101

Purpose

Phagemid that expresses LacI, and pIV under the Lac promoter

Depositor

Kevin Esvelt

Insert

pIV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJJS005

Plasmid

#188328

Purpose

PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mNeonGreen::Linker

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCFT4.2

Plasmid

#208807

Purpose

Expresses the fluorescent protein phiLOV2.1

Depositor

François-Xavier Campbell-Valois

Article

Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

Insert

phiLOV2.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mKOκ-EC18154

Plasmid

#137077

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pIA1363

Plasmid

#166861

Purpose

Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp8 (ORF1ab Synthetic)

Use

Tags

His-GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFBOT563

Plasmid

#40864

Depositor

Harold Gainer

Article

Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

Insert

Oxytocin promoter

Use

AAV

Tags

Expression

Bacterial and Insect

Mutation

Promoter

mouse oxytocin promoter

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-HIS-CBM-Xmod-DocIII

Plasmid

#91698

Purpose

CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-HIS-CBM-Xmod-DocIII

Use

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-pHuji

Plasmid

#61555

Purpose

Red fluorescent protein based pH indicator in bacterial expression vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHuji

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pET-21a-PE2-His

Plasmid

#201973

Purpose

Express His-tagged PE2 in bacteria

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

PE2-HIS

Use

Tags

His and c-myc NLS

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRSAN-QUE20C

Plasmid

#129316

Purpose

QUEEN ATP sensor for 20degC

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-20C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-1u

Plasmid

#13549

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol WT (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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