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Plasmid#111578PurposeExpresses ARH3 (amino acids 14-363) in E. coliDepositorInsertARH3 (ADPRS Human)
UseTags6xHis-Sumo and HAExpressionBacterialMutationdeleted amino acids 1-13PromoterT7Available sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36β
Plasmid#214344PurposeProtein overexpression in bacteriaDepositorInsertIL36B (IL36B Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36α
Plasmid#214343PurposeProtein overexpression in bacteriaDepositorInsertIL36A (IL36A Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PPP1CA_Metallophos
Plasmid#109934PurposeProtein expression and purification of PPP1CA_MetallophosDepositorInsertPPP1CA_Metallophos
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R401
Plasmid#138222PurposeExpresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R360
Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tus pGST-//
Plasmid#53305PurposeExpresses GST-tagged E.coli replication terminator protein Tus.DepositorInsertE.Coli Tus replication terminator protein
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCasTet-λ
Plasmid#128318PurposeConstitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.DepositorInsertTetR and pTetR/TetO
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationConstitutive expression of cas9 and anhydro-tetra…Promotertet promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Integrin Beta1-mPA-GFP
Plasmid#186302PurposeExpresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoterDepositorInsertIntegrin Beta 1 (ITGB1 Human)
UseTagsmPA-GFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Smurf2 wt
Plasmid#13504DepositorInsertSMURF2 (SMURF2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-hfYFP
Plasmid#186522PurposeHyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertHyperfolder YFP
UseTags6xHisExpressionBacterialMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
UseTagsGSGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-FLAG-Synaptojanin 1-145
Plasmid#22293DepositorInsertSynaptojanin 1_145 (SYNJ1 Human)
UseTagsFlag and GFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pMD18-T-pyrF
Plasmid#174388Purposemodified pMD18-T containing pyrF and its native promoter, AmprDepositorInsertpyrF
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC018 - LshCas13a from Leptotrichia shahii
Plasmid#91908PurposeExpresses LshCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLshCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pBA615
Plasmid#186239PurposeeLbuCas13a Negative Control (RFP targeting)DepositorInsertpTet-LbuCas13a + crRNA negative control
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only