-
Plasmid#136291PurposeExpresses Micrococcal nucleaseDepositorInsertMNase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a
Plasmid#113431PurposeExpresses 10xHis-MBP fused to LbCas12aDepositorHas ServiceCloning Grade DNAInsertLbCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Frb-v1
Plasmid#201923PurposeE. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.DepositorInsertsFrbA
FrbB
FrbC
FrbD
FrbE
UseTagsExpressionBacterialMutationPromoterT7 (modified), T7 (modified, moderate), T7 (modif…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn WT
Plasmid#36046DepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)
Plasmid#24859DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationFull length.PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSL-MS2-12X
Plasmid#27119DepositorInsertMS2 binding sites (12)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKD236-4b
Plasmid#90956PurposeExpresses ThsS constitutivelyDepositorInsertThsS
UseTagsExpressionBacterialMutationPromoterBba_J23104Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pQE30-HyPer7
Plasmid#141071PurposeBacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsHisx6ExpressionBacterialMutationPromoterT5Available sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_3xNLS_SpCas9_protein_expression
Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-42)
Plasmid#71875PurposeExpresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorInsertAbeta(M1-42) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTET GFP10-FRB/FKBP-GFP11
Plasmid#182497PurposeTripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coilsDepositorInsertsUseTagssplit GFP10 tripartite and split GFP11 tripartiteExpressionBacterialMutationPromoterTETAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE-4A6-vioC-3A2-vioD
Plasmid#73440PurposeViolacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.DepositorInsertPG6-vioABE-P4A6-vioC-P3A2-vioD
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6, P4A6, and P3A2 (orthogonal T7-lac variants)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV
Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsMBP-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-eGFP
Plasmid#32548DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pERM2-nhpSer
Plasmid#201922PurposeEnables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.DepositorInsertsSepRS(2)
Sep-tRNA v2.0
SerB
EF-Sep
UseTagsNoneExpressionBacterialMutationE412P, E414F, T417K, P495M, I496W, F529S and H67R…PromoterGlnS (constitutive), OXB20 (constitutive), lpp (c…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
UseTags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
UAS:TVA-mCherry
Plasmid#187823PurposeExpression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.DepositorInsertTVA-mCherry
UseTagsExpressionBacterialMutationPromoter5xUASAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only