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pLz37_CB_operon_CsoS2_N1-N4

Plasmid

#140856

Purpose

Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,5C6A-GFP

Plasmid

#80910

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

P5C6A and PM,5C6A

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pOSIP-KL (KanR, Lambda)

Plasmid

#45984

Depositor

Keith Shearwin, Drew Endy

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST17-TARG1

Plasmid

#172594

Purpose

For purification (from E.coli) of His-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET24_NusA-Tus

Plasmid

#165959

Purpose

Expression of soluble E. coli Tus protein

Depositor

Keith Pardee

Article

Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

Insert

Tus

Use

Tags

His-NusA-Tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pVRb01_syn20

Plasmid

#49704

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf01_syn20

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pJMP2832

Plasmid

#160672

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2820

Plasmid

#160669

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Venus-pBAD

Plasmid

#54859

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Use

Tags

6xHis and Venus

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pNCS mTangerine

Plasmid

#91763

Purpose

Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Tangerine

Use

Tags

no tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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HS69 pComb3xss anti-Homer1 nanobody

Plasmid

#134716

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFLxR5

Plasmid

#149482

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLuxB

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD33-mf-lon

Plasmid

#21867

Depositor

Robert Sauer

Article

Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

Lon (Mfl404 Mesoplasma florum)

Use

Tags

Expression

Bacterial

Mutation

all TGA codons changed to TGG codons

Promoter

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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Ftz

Plasmid

#11243

Depositor

Robin Reed

Article

Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

Ftz (ftz Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pQFT

Plasmid

#203674

Purpose

pQF derivative with promoter PQ5 exchanged for promoter PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

PQJ

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6*)-mCherry

Plasmid

#168048

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23101* and PBAD

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEcoFus

Plasmid

#187833

Purpose

1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpression

Depositor

Jörg Vogel

Article

Ponath et al Nat Microbiol. 2021 Aug;6(8):1007-1020.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-TEVP-cleavableELP

Plasmid

#219402

Purpose

Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

Depositor

Danielle Mai

Article

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

TEVP-cleavableELP

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pXD69m2-AphA

Plasmid

#191247

Purpose

E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYUB1062-GFP

Plasmid

#117695

Purpose

Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

Depositor

Elizabeth Fullam

Article

Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

Type

Empty backbone

Use

Tags

GFP and His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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