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pGEM-HE-Glyco-Venus-bPAC(S27A)
Plasmid#165490PurposeXenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activityDepositorInsertGlyco-Venus-bPAC(S27A)-Myc
UseCdna expression, xenopus oocyteTagsMyc and VenusExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Km
Plasmid#45938Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTagsHIS-6-tag and Twin-Strep-tagExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDF_LuxR
Plasmid#140039PurposeConstutitive expression of LuxR protein.DepositorInsertpLuxL and LuxR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-SBP-TEV
Plasmid#36943PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6xHistag-SBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
SARS_pNIC-CTB10H
Plasmid#153056PurposeExpression construct for producing Avi tagged Seryl-tRNA synthetaseDepositorInsertSARS (SARS1 Human)
UseTagsAviExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-eGFP-dspB(E184Q)-6xHis
Plasmid#175783PurposePlasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q)-6xHis
Plasmid#176576PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-PRC1
Plasmid#69111PurposeFor bacterial expression of recombinant human PRC1 tagged with His.DepositorInsertProtein Regulator of Cytokinesis 1 (PRC1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp R4-4 mutant
Plasmid#173491PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.DepositorInsertSfp-R44
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCis mCherry-SS Himar1 A7
Plasmid#84687PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJS002
Plasmid#188325PurposePCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::GFP::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pScaf-7560.1
Plasmid#111406PurposepScaf phagemid with insert for generating DNA origami scaffold 7560 bases in lengthDepositorInsertpScaf-7560.1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMyrPalm_mEGFP
Plasmid#21037DepositorInsertpMyrPalm
UseTagsmEPFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-Rad23A_pET11a
Plasmid#169124Purposeexpression of proteinDepositorInserthRad23A (RAD23A Human)
UseTagsExpressionBacterialMutationwtPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
P70a-deGFP-sc101-kan
Plasmid#184841PurposeExpression of deGFPDepositorInsertgreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpromoter OR2-OR1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold-TF-hRubicon
Plasmid#64147PurposeFor protein expression in E. coliDepositorInsertRubicon (RUBCN Human)
UseTags6xHis-tag and Trigger Factor (TF)ExpressionBacterialMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-K14R
Plasmid#185759PurposeBacterial expression of His6-Ubc9-K14RDepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationK14RPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET42b-HF-BE3
Plasmid#87438PurposeExpresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertHF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only
