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Plasmid#129570PurposeEmpty backbone for FLAG tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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pET11a-SUMO2
Plasmid#53142PurposeE.coli expression of untagged mature human SUMO2 (amino acids 1-92)DepositorInsertSUMO2 (SUMO3 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-92)PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
UseTags6x-Histidine tag, GFP, T7 leader sequence, and Thā¦ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilB
Plasmid#162845PurposeExpression of PilB with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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p109.1
Plasmid#44518DepositorInsertHistone 4
UseTagsGSTExpressionBacterialMutationcontains full length (aa 1-102)PromoterAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMTEZ-TeTxLC
Plasmid#32640DepositorInsertTetanus toxin light chain
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pMis1_1B
Plasmid#192079PurposeA pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent inductionDepositorInsertsKanR
rhaR
rhaS
pBBR1 rep
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL10-mRuby-iATPSnFR1.0 bb118
Plasmid#158980PurposeConstitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.DepositorInsertmruby-iATPSNfR1.0
UseTagsmruby3ExpressionBacterialMutationcodon optimized for e. coli from Addgene#102557PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.GĪ±11-LgB97
Plasmid#134361PurposeNanoluc complementation assay. Expression of GĪ±11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of GĪ±11. Addition of the HA epitope at N terminus of GĪ±11.DepositorInsertGĪ±11-LgB97 (GNA11 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
p15a-AsCas12f-blaZ
Plasmid#171609PurposeAsCas12f1-based E. coli genome editingDepositorInsertAsCas12f1
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pComb3HSS
Plasmid#64133Purpose2nd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTagsgeneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28C-SARS-CoV-2-Nucleocapsid
Plasmid#204402PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsidDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-60 Grx1-roGFP2-His
Plasmid#64799PurposeBacterial expression of His-tagged Grx1-roGFP2DepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsHis and roGFP2ExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP.R364
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His10-SHP1_PTPase (243-541aa)
Plasmid#180812PurposeExpression of 10*His tagged human SHP1 phosphatase domain (243-541aa)DepositorInsertSHP1_PTPase (243-541aa) (PTPN6 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV siteā¦ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGT-S1
Plasmid#122582PurposeMAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only