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pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9CyMutL
Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
(SIM)10
Plasmid#126946PurposeBacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human HUWE1 (3993-4374)
Plasmid#110754PurposeBacterial Expression of HUWE1 HECT domainDepositorInsertHUWE1 (HUWE1 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-mEGFP-SpyTag
Plasmid#72325PurposeBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.DepositorInsertpET28a SnoopTag-mEGFP-SpyTag
UseTagsN-terminal His6 tagExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEA183
Plasmid#187873PurposeExpresses eGFP under the virB promoter.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterAgrobacterium virB promoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
6MIW
Plasmid#127291PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorInsertHUWE1 (HUWE1 Human)
UseTags6xHis-TEV (MHHHHHHSSGRENLYFQG)ExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6T7
Plasmid#71462PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterhuman U6/phage T7Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FAM98A-Halo
Plasmid#175329PurposeProduction of HaloTag fusion proteinDepositorInsertFAM98A (FAM98A Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-3xHA-SAPAP3
Plasmid#190200PurposeExpresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytesDepositorInsert3xHA-SAPAP3 (Dlgap3 Mouse)
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-aHL_spytag_his
Plasmid#107334PurposeMonomeric SpyTag-6×-His alpha-hemolysinDepositorInsertalpha-hemolysin-SpyTag-6xHis
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CTV
Plasmid#131590PurposePlasmid for the targeting of chloramphenicol geneDepositorInsertNeomycin resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
VV020: WT Arch-3 - eGFP in pET28b
Plasmid#58488PurposeExpresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3
UseTagseGFPExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMP2463
Plasmid#107774PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pBBR1-mcs5
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pN006
Plasmid#188470PurposeLentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)DepositorInsertAsCas12a
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
