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Plasmid#18880DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046785
Plasmid#145684PurposeBacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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CTV
Plasmid#131590PurposePlasmid for the targeting of chloramphenicol geneDepositorInsertNeomycin resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6T7
Plasmid#71462PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterhuman U6/phage T7Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
VV020: WT Arch-3 - eGFP in pET28b
Plasmid#58488PurposeExpresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3
UseTagseGFPExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
(SIM)10
Plasmid#126946PurposeBacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS Galectin1
Plasmid#19216DepositorInsertGalectin1 (LGALS1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
Human HUWE1 (3993-4374)
Plasmid#110754PurposeBacterial Expression of HUWE1 HECT domainDepositorInsertHUWE1 (HUWE1 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-aHL_spytag_his
Plasmid#107334PurposeMonomeric SpyTag-6×-His alpha-hemolysinDepositorInsertalpha-hemolysin-SpyTag-6xHis
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FAM98A-Halo
Plasmid#175329PurposeProduction of HaloTag fusion proteinDepositorInsertFAM98A (FAM98A Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-143
Plasmid#118559PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-mEGFP-SpyTag
Plasmid#72325PurposeBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.DepositorInsertpET28a SnoopTag-mEGFP-SpyTag
UseTagsN-terminal His6 tagExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pN006
Plasmid#188470PurposeLentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)DepositorInsertAsCas12a
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T-2/hGGA3(GAT)
Plasmid#79436PurposeExpresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coliDepositorInsertGGA3 (GGA3 Human)
UseTagsGSTExpressionBacterialMutationamino acids 107-286PromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only