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Plasmid#214051PurposeEmpty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET-Duet1_6xHis-TEV-eGFP-P62
Plasmid#190929PurposePlasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390DepositorInsertP62 (SQSTM1 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
K560GFP_17NL
Plasmid#129767PurposeExpresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assaysDepositorInsertKHC (Khc Fly)
UseTagsGFP and His6ExpressionBacterialMutationDAL insert before A345PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGDP2 MCR-1
Plasmid#118404PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.DepositorInsertMCR-1
UseTagsExpressionBacterialMutationPromoterpLacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mScarlet-I
Plasmid#163756PurposeExpresses Streptomyces codon-optimised mScarlet-IDepositorInsertmScarlet-I
UseSynthetic BiologyTagsFlag and FlAsH tagsExpressionBacterialMutationPromoterSP44Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII TEV LIC cloning vector (1R)
Plasmid#29664DepositorTypeEmpty backboneUseTagsStrep II-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP2a-Halo
Plasmid#175309PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP2 (IGF2BP2 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Kan-LacI
Plasmid#79826PurposeArabinose induced expression of Lac repressorDepositorInsertLacI
UseTagsHis6ExpressionBacterialMutationPromoteraraBpAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
UseTagsExpressionBacterialMutationPromoterSpectinomycin resistance gene promoter and noAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2B-58
Plasmid#177928PurposeExpresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)DepositorArticleInsertEGFP
UseTagsExpressionBacterial and YeastMutationPromoterTET4 (contains four rtTA binding sites)Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-Geo_st
Plasmid#87700PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBPDepositorInsertGeoCas9
UseTags10xHis-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QNA
Plasmid#129305PurposeNegative control for ATP measurementDepositorInsertQUEEN-NA
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-1xGFP-6xHis
Plasmid#196972PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTags6xHis and GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-mpeg1-dendra2
Plasmid#51462Purposeexpresses photoconvertible dendra in macrophagesDepositorInsertmpeg-dendra
UseTagsExpressionBacterialMutationPromotermpeg1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZA16mflon
Plasmid#75439PurposePlasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.DepositorInsertmf-lon
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
BPK2014-AaCas12b
Plasmid#121949PurposeE. coli expression, protein purificationDepositorInsertAaCas12b
UseTags10xHis tagExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_FLAG-pvc2
Plasmid#198285PurposeContains an external epitope tag on the sheath protein; for immunofluorescence-based binding assaysDepositorInsertPVCpnf_FLAG-pvc2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pST-2K
Plasmid#44564Purposefor ectopic constitutive expression of two mycobacterial proteins in one vectorDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP
Plasmid#165090Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGex-4T-2_GABARAP
Plasmid#73948PurposeFor recombinant expression of human GABARAP in E.coliDepositorInsertGABARAP (GABARAP Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only