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Plasmid#176228PurposeA template plasmid for amplification of the cI-hok-strA cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, strA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1950
Plasmid#205983PurposeGa0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DRDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array
UseTags14xHis and TwinStrep-tagExpressionBacterialMutationPromoterT7 (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXY027
Plasmid#98915PurposeExpress FtsZ tagged with GFP in E coliDepositorInsertFtsZ
UseTagsGFPExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFAK2 (PTK2B Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDB179
Plasmid#91960Purposeempty expression vector for 10His-SUMO tag protein purificationDepositorTypeEmpty backboneUseTags10-His and Smt3ExpressionBacterialMutationPromoterT7Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB045a
Plasmid#79224PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTet-tns
Plasmid#170628PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal no NLS
Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterD-mannoseAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU
Plasmid#64180Purposeharbours marker gene URA3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertapmA (apmA Staphylococcus aureus)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJJS005
Plasmid#188328PurposePCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mNeonGreen::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 (XSB363)
Plasmid#78505PurposeConstruct for expressing Grb2 in E. ColiDepositorInsertGrb2 (GRB2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBB2
Plasmid#80749PurposeExpression of human beta B2-crystallin in E. coliDepositorInsertCRYBB2 (CRYBB2 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP
Plasmid#165090Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS124
Plasmid#207593PurposeGFP-BIB H20A expressionDepositorInsertGFP-BIB H20A
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDWJ27
Plasmid#207604PurposeGFP-BIB expressionDepositorInsertGFP-BIB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
UseTagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alaninePromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only