-
Plasmid#34957DepositorInsertPSmOrange2
UseTagsExpressionBacterialMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …PromoterAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
SL-SunTag
Plasmid#196919PurposeThe SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loopDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PtxS1-c003
Plasmid#173076PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPtxS1
UseTagsExpressionBacterialMutationD35-I221PromoterAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7
Plasmid#41187DepositorInsertS. cerevisiae Hsp82
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
pET28a-SdrG_N2N3-HIS-ybbr
Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SUV420H2
Plasmid#32864PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSUV420H2 (KMT5C Human)
UseTagsHis tag (with TEV protease)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
CL7_2xNLS_iGeoCas9(G)_2xNLS_His
Plasmid#222989PurposeBacterial expression of iGeoCas9(G) constructDepositorInsertCL7_2xNLS_iGeoCas9(G)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM
Plasmid#221379PurposeExpresses FANCM in bacterial cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PDCD6_EF-hand
Plasmid#110009PurposeProtein expression and purification of PDCD6_EF-handDepositorInsertPDCD6_EF-hand (PDCD6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pET-6xHis-(pos9)GFP
Plasmid#89247PurposeExpresses GFP with +9 theoretical charge in E.ColiDepositorInsertHis6-(pos9)-GFP
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.0-Pct5.1-crRNA(AarI)
Plasmid#191633PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
Mut2-RS
Plasmid#182882PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut2-RS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-MBP
Plasmid#112568PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-MBP and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PSAT1
Plasmid#83908Purposestable overexpressionDepositorInsertphosphoserine aminotransferase 1 (PSAT1 Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only