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Showing: 361 - 380 of 28122 results

  4. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Use
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  7. pET21a-alpha-synuclein

    Plasmid
    #51486
    Purpose
    Inducible expression of human alpha-synuclein in E.coli
    Depositor
    Michael J Fox Foundation MJFF
    Insert
    alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    51486-plasmid-map-sequence-id-139447

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  8. pSAB35

    Plasmid
    #175787
    Purpose
    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag
    Depositor
    John Rubinstein
    Article
    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246
    Insert
    SidK (1-278)
    Use
    Tags
    3xFLAG and 6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    175787-plasmid-map-sequence-id-348166

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  12. pGM1192

    Plasmid
    #115683
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    mCherry, aac(3)IV, tsr
    Use
    Tags
    Strep-tagII on mCherry, codon optimized
    Expression
    Bacterial
    Mutation
    mCherry codon optimized for Streptomyces
    Promoter
    SF14*
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    115683-plasmid-map-sequence-id-237128

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  13. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Article
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
    Use
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169815-plasmid-map-sequence-id-334563

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  14. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Depositor
    Christopher Johnson, Gregg Beckham
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112202-plasmid-map-sequence-id-334619

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  19. pST44-AP-1 core

    Plasmid
    #184809
    Purpose
    Bacterial expression of clathrin adaptor AP-1 core complex
    Depositor
    James Hurley
    Article
    Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10
    Insert
    Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
    Use
    Tags
    GST tag and His6
    Expression
    Bacterial
    Mutation
    AP1S3: S148C; AP1B1: K359R, E476K
    Promoter
    T7
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    184809-plasmid-map-sequence-id-365328

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Showing: 361 - 380 of 28122 results