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Showing: 3721 - 3740 of 28413 results

  1. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid
    #111850
    Purpose
    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12V (KRAS Human)
    Use
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Valine
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111850-plasmid-map-sequence-id-213464

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  2. pSLC-254

    Plasmid
    #124291
    Purpose
    Expression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.
    Depositor
    Swaine Chen
    Article
    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4
    Insert
    vsfGFP-0
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    124291-plasmid-map-sequence-id-243082

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  7. pWT004a

    Plasmid
    #107883
    Purpose
    R1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria
    Depositor
    David Liu
    Article
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    PBAD-EGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107883-plasmid-map-sequence-id-207299

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  9. pET24a-VHH-2xTS

    Plasmid
    #109419
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109419-plasmid-map-sequence-id-211590

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  14. roGFP_iE_C147S_pQE30

    Plasmid
    #48631
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Use
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, C147S
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48631-plasmid-map-sequence-id-70379

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  15. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα12-LgB115 (GNA12 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

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Showing: 3721 - 3740 of 28413 results