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Plasmid#118225PurposeEmpty E. coli - B. burgdorferi shuttle vector, gentamicin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only
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p404TDH3
Plasmid#15973DepositorInsertTDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationTDH3 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-Trx-mSA2
Plasmid#52320PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, S tag, TEV site, and ThioredoxinExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EC12WTC9AAvi
Plasmid#47925PurposeExpresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.DepositorInsertE-Cadherin EC12
UseTags6xHis, Avi Tag, and TEV CleavageExpressionBacterialMutationchanged Cysteine 9 to AlaninePromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human PP2A B56α full length
Plasmid#132635Purposeexpresses human PP2A B56α full length in E. coliDepositorInsertPP2A B56α
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE31-rsEGFP2
Plasmid#102879PurposeExpresses rsEGFP2 in bacterial cellsDepositorInsertrsEGFP2
UseTags6xHisTagExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110.i1
Plasmid#135481PurposeE. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110.i1
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUC01
Plasmid#177204Purposechromogenic reporter for gene knockout in StreptomycesDepositorInsertSaindC
UseSynthetic BiologyTagsNONEExpressionBacterialMutationNONEPromoterTGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonorGFP(Ptr)
Plasmid#175578PurposeDNA donor for transpositionDepositorInsertMini-Tn, GFP cassette
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM1s3ATScsg-Etag
Plasmid#137946PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expressionDepositorInsertsSynthetic curli operon
Synthetic curli operon
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterpTlpA36Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlc_N + pSiMPlc_C
Plasmid#134312PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertcmR_gp41-1 N-intein + cmR_gp41-1 C-intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hisMBP-AsCpf1-EC
Plasmid#79007PurposeExpressing his-MBP tagged AsCpf1 (E.coli codon optimized)DepositorInsertAsCpf1
UseTags6xHis-MBP-NLS and NLS-HAExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-167
Plasmid#118579PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-ER-MT-P2A
Plasmid#214892PurposeExpresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sitesDepositorInsertSPLICSFAST-ER-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpySrtA
Plasmid#51139Purposeexpression plasmid for N-terminal his tagged S.pyogenes SrtADepositorInsertS.pyogenes SrtA
UseTags6x HisExpressionBacterialMutationDeleted amino acids 1-80PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
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evoFERNY-Cas9NG
Plasmid#203329PurposePlasmid for bacterial purification of codon optimized evoFERNY-Cas9NGDepositorInsertevoFERNY-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRha-ABE8e-SpCas9-NG
Plasmid#201190PurposeExpresses the base editor ABE8e-SpCas9-NG in bacterial cellsDepositorInsertABE8e-SpCas9-NG
UseTags8xHIS and BP-NLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLBAD
Plasmid#32055PurposeVector optimized for inducible gene expression in BurkholderiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPbadAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PABPC1_PABP
Plasmid#109931PurposeProtein expression and purification of PABPC1_PABPDepositorInsertPABPC1_PABP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only