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pSH424-ssr
Plasmid#198026PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Sm resistance cassette
UseTagsExpressionBacterialMutationPromoterSmR promoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
HS69 pComb3xss anti-Homer1 nanobody
Plasmid#134716PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))
UseTags6xHis-HAExpressionBacterialMutationPromoterlacAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-B mCherry
Plasmid#108857PurposeEncodes for the fluorescent protein mCherryDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
p7XC3H
Plasmid#47065PurposeFX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEE050
Plasmid#176807PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert601
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
UseTagsStrep(II)-tagExpressionBacterialMutationPromoterT7 PromotorAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pScaf-7560.1
Plasmid#111406PurposepScaf phagemid with insert for generating DNA origami scaffold 7560 bases in lengthDepositorInsertpScaf-7560.1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
p1153
Plasmid#222930PurposeShuttle vector carrying a Candida auris centromere (CEN 4) with Nourseothricin selectable markerDepositorInsertCEN 4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBluescript-Evf1
Plasmid#99479PurposeSubcloning of the Evf1 long non-coding RNADepositorInsertEvf1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialMutationPromoterT7lacAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-con-GFPuv
Plasmid#106386PurposeConstitutive trc promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1
Plasmid#187936PurposePlasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327DepositorUseTagsGST-mCherryExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSR348
Plasmid#124713PurposeExpression of narX under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS193* - LacI is truncated and likely deactivated.…PromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iL
Plasmid#83313PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iL
UseTags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iL betwe…PromoterT5Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
SBFP2-anti-AlfaTag nanobody
Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only
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